Study On The Technique In Vitro Of Mornordica Charantia (Lvren)

A study was conducted to establish a rapid propagation technique system using shoot-tips and stem segments of in vitro seedlings of Mornordica charantia L (Lvren) . The main objects of the study were shoot induction, shoot proliferation, and plantlet rooting. The results were as follows:(1)The medium MS+2mg/L BA+0.01mg/L IBA was suitable for shoot induction, resulting in 41.92% of shoot regeneration rate.(2)MS+0.5mg/L KT+0.5mg/L BA+0.05mg/L IBA was suitable for multiplication. (3)The suitable sugar for multiplication was sucrose. The granulated cane sugar was not as good as sucrose, but it could reduce cost, and was suitable for commercial production.(4)A higher lever of iron salt was demanded for multiplication, the best concentration being 15 mg/L.(5)The nitrogen forms and combinations had important effect on multiplication. MS added with 20 mg/L NH₄NO₃, KNO₃, 0.5 mg/L BA , 0.5 mg/L KT and 0.05mg/L IBA was suitable for multiplication, resulting in 100% of shoot regeneration rate and a multiplication rate of 4.78.(6)ABA had restraining effect on shoot regeneration. Young plants died down resulting in a lower and lower average multiplication rate with the increase of ABA concentration.(7)AgNO₃ could stimulate shoot regeneration. The suitable concentration for multiplication was 5mg/L, resulting in a multiplication rate of 3.45. The addition of AgNO₃ to the medium could inhibit callus formation. Fewer calluses were formed while the concentration of AgNO₃ increase.