Study On Micropropagation Of Three Species Of Gesneriads

Gesneriaceae has many varieties. Some of them live specially in China and are dry resistant, humid resistant and shade resistant. Some species of gesneriads are stub-shaped or leaf-shaped. There are rich shapes of flower and many flowers are worthy of appreciation since they are marvelous and florid. But most of them are lying remote mountains and unknown to people and not developed commercially yet, or many species of gesneriads are close to extermination due to descendiblity defeat or excess development by people although they are worthy of appreciation. So it is great significant to study on tissue culture and rapid propagation of Gesneriads, it will be also great helpful to commercial production of gesneriads and in vitro conservation of the plant germplasm.The study takes three species of gesneriads, Chirita eburnean, Chirita fimbrisepala, Chirita pungentisepala, whose are all originated in Guangxi, China, as experimental material to establish the micropropagation system of gesneriads. The pedicel is the best suitable explant for initial culture, while the most optimal medium of the sprouting of three species is MS + NAA0.1mg/L + 6-BA0.1mg/L + suhar30g/L + arga6g/L. The effect of subculture MS medium consists of NAA and 6-BA ( both are 0.1 mg/L) is best for Chirita eburnea and Chirita pungentisepala, and Chirita fimbrisepala had more adventitious shoots formation in MS medium supplemented with 0.1 mg/L NAA, 6-BA0.05 mg/L. The best medium for the rootage of plantlets of Chirita eburnean and Chirita pungentisepala is 1/2MS +sugar 10g/L + arga6g/L; 1/2MS + Ac3g/L + sugar10g/L + agra6g/L is the best suitable rooting culture medium of Chirita fimbrisepala. The results showed that, three species of gesneriads are all suitable to tissue culture and rapid propagation. When optimum levels of plant growth regulating substance, are utilized, three species of gesenriads could be regenerated 1500-2500 plantlets from a section of 5-8cm pedicel. The technique is practically suitable for commercial production of potted flower of gesneriads.