Compare The Effects Of 15 Kind Of Herbal Medicine To Inhibit Toxoplasma

Objective: To investigate the new herbal medicine which can inhibit Toxoplasma, Thisexperimentation use in vivo experiment and in vitro experiment to select out the effectivemedicine from 15 kind of herbal medicine such as HuangQin and GanCao, use those effectivemedicine make up composite medicine, then eompare, the effects of composite herbal medicine toconfirm the best composite herbal medicine.Select out the medicine which can inhibit Toxoplasma gondii from15 kind of herbalmedicine. Methods: In vitro experiment: Using the tachyzoite ofToxoplasma gondii RH straininfect the Vero cell then observe the cell condition, in taipanblue staining experiment calculate theToxoplasma death rate and restrain rate. Mice infected Toxoplasma experiment, observe the micefettle, note every group mice death time and number. Results: HuangQin, GanCao, BaiBu,GuanZhong, BingLang, YaDanZi, HuangQi can treating mice acute Toxoplasmosis.Use those effective medicine make up composite herbal medicine .compare the effects of 4composite herbal medicine. Methods: the method of in vitro experiment same as front. In vivoexperiment include Mice infected Toxoplasma experiment, serum NO level experiment, Liver andspleen pathological section. Results: Composite herbal medicine could inhibit- the growth andreproduction of Toxoplasma, this effects of composite one (consist of BinLang, HuangQin,HuangQi, GanCao) and composite two(consist of YaDanZi, HuangQin, BaiBu, GanCao)werebetter than single herbal medicine. Mice infected Toxoplasma experiment, composite herbalmedicine group and control group could treating mice acute Toxoplasmosis, composite one wasbetter than HuangQin obviously. In serum NO level experiment, the mice serum NO level ofcomposite one and four group higher than HuangQin group obviously, composite two and threecompare with HuangQin non-obvious difference. In Liver and spleen pathological sectionexperiment, composite herbal medicine could Protects the liver and spleen of Toxoplasmosis mice.The above test result indicated that, HuanGqin, GanCao, BaiBu, GuanZhaong, BingLang,YaDanZi could inhibit the growth and reproduction of Toxoplasma in vitro. HuanGqin, GanCao, BaiBu, GuanZhaong, BingLang, YaDanZi could treating mice acute Toxoplasmosis. compositeherbal medicine could inhibit Toxoplasma no matter in vitro or vivo, the inhibit toxoplasmaeffect of composite one was battter than single medicine.