Fingerprint And Quality Evaluation Of Salvia Miltiorrhiza Bunge

To strengthen the quality control and comprehensive utilization of S. miltiorrhiza, the samples that different parts, different sources, different harvested time and different kinds of roots of S. miltiorrhiza were evaluated by HPLC fingerprint combined with quantitative analysis of multiple components. The standard fingerprints of cardiotonic pills and S. miltiorrhiza from Shangluo were constructed. The main conclusions were following:1. Reproducibility and methology of fingerprintThe results of mathology test conformed to requirements for studdying fingerprint of TCM injections(draft). The small changes of extraction and chromatographic conditions had little influences on fingerprinting similarity. The method in the experiments could be used to constructed the fingerprints of liposoluble and water-soluble components in S. miltiorrhiza.2.Fingerprint of cardiotonic pill and S. miltiorrhiza from ShangluoThe standard fingerprints of cardiotonic pills and S. miltiorrhiza from Shangluo were constructed with the average method of common pattern. Quantitative analysises of multiple marker compounds were also used for quality evaluation. It was found the quality of TCM was not evaluated comprehensively only by fingerprint or quantitative analysis. Chromatographic fingerprint together with quantitative analysis of multiple marker compounds was a good method for quality assessment of TCM.3. Quality of S. miltiorrhiza from different habitatsThe contents of liposoluble components in Salvia castanea Diel f. tomentosa Stib were high. tanshinoneâ…¡A content was 1.62%, 4.47 times more than that in S. miltiorrhiza.The number of peaks of water-soluble components in Salvia castanea Diel f. tomentosa Stib was less and content of salvianolic acid B was lower than that in S. miltiorrhiza. But the area of peak 9 which was concluded to be rosmarinic acid was 8 times more than than in S. miltiorrhiza. The peak numbers of liposoluble and water-soluble components in S. miltiorrhiza from other provinces was the same. The best quality of S. miltiorrhiza was from Shandong province,the next was from Shangluo in Shasnxi province and Anguo in Hebei province and the worst was from Beijing,Anhui and Jiangsu province.4. Distribution and dynamic accumulation of active components in S. miltiorrhizaTanshinoneâ…¡A was the principal one of liposoluble components which were mainly contained in root-periderm. salvianolic acid B was the principal one of water-soluble components which were mainly contained in xylem parenchyma and Vascular cylinder. But Danshensu was more in the leaves than in other parts. Contents of some water-soluble components were also high in flower and receptacle. and the active components in S. miltiorrhiza and S.miltiorrhiza f.alb. was the same on distribution but different on the content. The dynamic accumulation of liposoluble components in S.miltiorrhiza and S.miltiorrhiza f.alb. was decrease-increase-decrease-increase from may to december. A climax was reached in august. The dynamic accumulation of water-soluble components was not the same in the two kinds of S.miltiorrhiza. However, a climax was also reached in august and a rising trend was found after october in them.5. Quality of different kinds of roots of S.miltiorrhizaRoot rot and nematode disease which influenced the quality were the common disease of S. miltiorrhiza. The active components were more seriously influenced by root rot than by nematode disease. The accumulation of dihydroxytanshinoneâ… and peak 6 in chromatogram of liposoluble components was promoted by root rot. That of tanshinoneâ…¡A and peak 7 was promoted by nematode disease. The contents of water-soluble components were reduced by the two disease.The quality of red root was the best. Contents of some liposoluble components were the highest but that of all the water-soluble components were the lowest in brown root. Light yellow root had the lowest contents of liposoluble components and lower contents of water-soluble components than red root.The quality of S.miltiorrhiza growing for one, two and three years was compared. It was the lowest for the liposoluble components contents in two years old of S.miltiorrhiza. Some liposoluble components increased greatly three later. The contents of water-soluble components did not increase with the age increasing except peak 6 and 9. The roots of S.miltiorrhiza were divided into 7 groups which were toot head, more than 0.8 cm for diameter, 0.5 to 0.8 cm, 0.3 to 0.5 cm, 0.2 to 0.3 cm, 0 to 0.2 cm and fibrous root. Evaluated on the liposoluble components, the roots of S.miltiorrhiza for diameter between 0 to 0.2 cm were the the first grade, the second grade was between 0.5 to 0.8cm, the third was between 0.3 to 0.5 cm and more than 0.8 cm, the forth was between 0.2 to 0.3 cm and fibrous root,the last was root head. Evaluated on the water-soluble components, the thicker the root was, the better the quality was. The first grade was more than 0.8 cm, the second was between 0.2 to 0.8 cm, the third was between 0 to 0.2 cm and root head, the last was fibrous root.