Studies On The Anti-epileptic Effects And Mechanisms Of Ginseng-Tatarinowii Docoction

The antiepileptic effectiveness and its meeanisms of GTD werestudied in thes paper The work was conducted in four epileptic medls:maximal electroshock(MES), metrazol seizure test(MST), strychinseizure test(SST) and pilocarpine induced seizure. The experiment resultsindicated that:l. The median convulsive dose of pentylenetrazo, strychnine andelectrostdimulation were significently elevated by administration of GTD, italso improved the median lethal dose of pentylenetrazol and strychnine~ inmice, compared with that of the cotrol group(p <0.05 or p <0.0l);And it has a excellen effect against the pilocarpine induced acute statusepilepticus.2. In the model of pilcarpine-induced seizure, the results show thatGTD has no inflences on amino-transmitteres in centrol nerve systems ofbreely moving rats; It can inhibite glutamate from overrelasing andincrease extracellular GABA concetration in the forebrain to the ratswhich were premedicated with 4g/kg GTD when they were administerPilocarpine; It can markdly increase GABA concentalon, though itdoesn't decrease the elevated glutamate level to the convulsive rats. Themeasured results by means of high perfance liquid chromatographymethod show:The forebrain concentration of ASP. Glu. GABA and Gly Of ratsadministrated GTD are 4.23± l.38, 9.l6±3.0l, 2.55±0.9l, 2.09±0.64μmol· g(-1) brain wet weight respectively, there are no different from that ofthe control group, they are 3.61 ± 0.51, 8.53±0.79, 2.04±0.5l,- 3-*reAR#at4$E$#*a @t$&texl.43ed.23pmol. g--' for Wewegh rewtly (P < 0.05);ComPared with the contrOl groUP, the Gfu in the groU ~catalwitu om dsch or Ptw in ratsMdiM with 4the W resul in a conditon that is aP tO snd is9.07 t l.33Pmol. g--' tw we weight there is a swifieeni he inGABA (3.1l l 0.62Pmol. g--' bdri we weight l50O/, mp.05), bot nochangr abou the levels ofglutama to>0.05);To eds ofltheic status rpilghcus PIDded by PilOCarpthe -Mtw GTD and saline wt a swifiCen bo ingluhae levels was obserVedll.29 l l.80 and l2.67 l 3.07Umol. g'bo we weigh comPared with tha of the conbol groU,p < 0.05); weobserVed no changr in GABA of the latert.04 l 0.7lPmol. g--' bdri weweigh0, a markedl eleVatio tO the former (3.20 l 0.50llmol. g--, bowewegh).mstoogical examinaon ofhiPPocamPUS revealed that: ndld twin the of GTD, whereas sem damags wh for netwdegeneIaon were noted in admhasthaon of salin.The results of Present StUdy demwt that GTD has ~anticyilghc effeets accothe to the bo of the foUr ePilghc medls,the levels of amin acid of the and the findin of hiStOfogicalexaminaon of hiPPOCaInPUS. the tw can d~ bo theconCeIhaon of GABA, enhanCe the inhibition of GABA, inhibit thegluthe from overmleasing, and decmp exhaocty of Glu,therefore achieve its antiedePtic action, We the neurons inhiPPOCamPUS were ProteCted frOm daInag.