The Experimental Research Of Chronic Osteomyelitis Extractant From Bone Marrow Decoction

Objective: To observe the effects of Bone Marrow Decoction(BMD) on chronic osteomyelitis rat models and investigate the effects of mechanismMethods: 46SD rat animals, half male and half female, were anesthetized by ketamine hydrahlaride. Suspend liquid of MRSA, which was passaged and recoveyed toxicity mixed with 5% sodium morrhuate, was injected into the tibual marrow of rats. 4 weeks later, chonic osteomyelitis was comformed from the X-ray. There were irrespectively given BMD, douche and oral medication, BMD douche, amikin and physiological saline. Chronic osteomyelitis model SD rats animals were randomly divided into four groups: BMD group â… ,BMD groupâ…¡, control group and blank group. A11 were treated for 1 month. The comparative observation of model animals were taken through bone X-rays, WBC, Leukocyte rolling, erytllrocyte c3b receptor ratio(E-c3bRR), erythocyte immune compound ratio(E-ICR) , Serum lysozyme activity , roentgenography histological ultastructral observations and electron microsope observations at the fixed time.Results: Great significant contrast were found among the results of BMD group I, BMD group , the blank group and those of the blank group(P<0.01); Contrast the results of extractant from BMD group with the control group, great diffences were made clear in respects of blood leukocyte count,E-c3bRR, E-ICR and serum lysozyme activity(P<0.01); The results of BMD group 1 were better those of BMDgroup â…¡, however, there were no statistics significance(p>0.05). From the X-ray , the bone external surface of BMD group â… , â…¡ are more smooth, bone vein are very uniform. As for bone marrow group â… , â…¡, there are many osteoblast and osteocyte on the surface of bone tissue. The hyperplasia of hematopoietic cell was very active, BMD groups were better than the control group and the blank group. In respects of roentengography and histological manifestation, BMD groups were better than the control group and the blank group.Conclusions: Extractant from BMD has good effects on chronic osteomyelities rat models, which may be related to its improvement of the organism immune function, the histological microenvironment and repairs of bone.