IL-10 And IFN-γ Level In Expressed Prostatic Secretion Of Chronic Prostatitis

Objctive: We studied the role of pro-inflammatory cytokine interferon-gamma (IFN-γ) and anti-inflammatory cytokine interleukin-10 (IL-10) in the process of CBP(Chronic bacterial prostatitis) and CPPS(Chronic pelcic pain syndrome), investigating whether the chronic inflammation and pain experienced by patients may be caused by an imbalance of pro-inflammatory versus anti-inflammatory cytokines and the type of immunity in region of prostate and analyzed the relationship between the cytokine level and number of WBC in EPS and the symptms of pain severity and the role of related cytokines in the process of CP.Methods: Sixty-eight men seen in the clinic of second hospital of Shandong university were collected to participate in the study. Patients evaluation included a complete medical history, a physical examination, and a two-glass urinalysis with cultures of urine and enumeration of leukocytes in EPS. At the same time, patients were asked to complete NIH-CPSI questionnaires.Prostatic fluid was obtained by prostatic massage and collected in a Eppendrof tube and stored at -80℃ immediately until used for cytokines analysis. The remaining fluid on the glass slide was placed under a coverslip and examined for WBCs in five fields at high power (400×). The average number of WBC/per high power field (hpf) was recorded. Cultures of urine of all patients and normal male controls were done on blood and MacConkey agar plates. Standard microbiologic methods were used to identify the microorganisms. Patients were classified into 4 categories according to two-glass test (PPMT) and NIH diagnostic criteria for chronic prostatitisxhronic bacterial prostatitis (NIH category Ⅱ, CBP) (n = 8), inflammatory CPPS (NIH category Ⅲa) (n = 28), noninflammatoryCPPS (NIH category Illb) (n= 16). The control group included 16 men with no lower urinary tract symptoms, palpably normal prostates, less than 5 WBC/hpf in EPS 0 IFN-y and IL-10 levels were measured in EPS by using enzyme-linked immunosorbent assay (ELISA). These assays are specific for IFN-y or IL-10 with a minimum detectable dose of 7 and 30 pg/mL, respectively. Statistical analyses were performed using Statistical Package of SPSS 12.0. Data were analyzed using t test,One-Way ANOVA test process and pearson correlations analysis.P values<0.05 were considered significant.Result: A statistically significant difference between the control group versus CBP and Ilia (PO.05) groups was found for IL-10 and IFN-y. The levels of IL-10 and IFN-y in EPS were statistically higher in CBP and Ilia CPPS than those in controls respectively.The ratio of T2/T1 distinction in Ilia CPPS and normal man was no statistically significant. In EPS of the patients with CP, there was a directly correlation between the IL-10 level and the score with NIH-CPSI about symptms of pain severity or discomfort. No significant correlation was found between the number of leukocytes in EPS and IL-10 or IFN-y level in all groups.Conclusions: In the EPS of man with CBP and Ilia CPPS, proinflammatory interferon- y and anti-inflammatory interleukin-10 levels were elevated in in our study.The two cytokines might be considesed as mediators of inflammatory response in prostate in patients with CP. The CAP has nothing to do with the imbalance of the Th2/Thl,both cell immunity and humoral immunity possibly enhance in local of prostate. In patients of CP, the level of IL-10 in EPS correlated directly with the symptoms of pain or discomfort severity. No correlation between the IL-10 or INF-y level and the number of leukocytes in EPS.