Abnormal Expression Of TRAIL Receptors In Decidual Tissue Of Chlamydia Trachomatis-infected Rats During Early Pregnancy Loss

Part ? Correlation between Chlamydia trachomatis,Ureaplasma urealyticum and infertilityObjectives:To investigate the relationship between Ureaplasma urealyticum(UU)and Chlamydia trachomatis(CT)genital tract infection and infertility.Methods:482 infertile patients in the reproductive center of Jinzhou First People's Hospital from January 2015 to December 2016 were selected as infertility group(91pairs of couples),and another 842 cases of healthy physical examination of the same period randomly were selected into control group.Fluorescence quantitative PCR was used to detect Ureaplasma urealyticum and Chlamydia trachomatis DNA in patient's genital tract secretion samples,and SPSS software was used for statistical analysis.Results:Infection rate of UU in infertility group and control group were 61.4%and40.9%(x~2=51.865,P<0.05)respectively,and CT infection rate in infertility group and control group were 5.4%and 2.3%(x~2=9.191,P<0.05).Compared to healthy control group,infertile patients had higher infection rate of Ureaplasma urealyticum and Chlamydia trachomatis.The UU infection rate of husband and wife in 91 couples of infertile group were 15.4%and 48.4%respectively,and there was no correlation between the UU infection in the husband and wife(r=0.075,P>0.05).The CT infection of husband and wife in 91 couples were 9.9%and 11%respectively,and the CT infection between husband and wife were correlated(r=0.707,P<0.05).Conclusion:There is a certain correlation between UU and CT infection of reproductive tract and infertility,therefore,monitoring UU and CT infection could contribute to the diagnosis of infertility and provides guidance for the treatment of infertile patients.Part ? Abnormal expression of TRAIL receptors in decidual tissue of Chlamydia trachomatis-infected rats during early pregnancy lossObjective:Chlamydia trachomatis is a general term for pathogenic microorganisms that can cause reproductive tract infections and are associated with spontaneous abortion.In this study,the expression of tumor necrosis factor-related apoptosis-inducing ligand(TRAIL;a cytokine related to cell apoptosis)and their receptors were detected in the decidua of the early pregnant rats after Chlamydia trachomatis infection in order to study their potential role in spontaneous abortion in early pregnancy caused by Chlamydia trachomatis infection.Method:Healthy adult female rats were selected and randomly divided into infection group and uninfected group.Infected group was successfully infected with vaginal inoculation of Chlamydia trachomatis strain D,and the uninfected group was inoculated with the same amount of normal saline,making the two groups of rats successful in pregnancy.On the 5th,6th and 7th days of pregnancy,the embryo implantation level of the infected and non-infected groups was observed;the expression of TRAIL and its receptors in decidua tissue were detected by Western Blot,PCR and immunohistochemical method on the 5th,6 and 7 days of pregnancy in infected group and uninfected group.Results:On the 6th and 7th days of pregnancy,the number of embryos implanted in the infected group was significantly reduced compared with the uninfected group on the same day(P<0.001);On the 5th day of pregnancy,there was no significant difference in the number of embryos implanted between the uninfected group and the infected group;When compared within the group,there was no significant difference in embryo implantation rates between the uninfected group and the infected group.On the 5th,6th and 7th day of pregnancy,the expression of TRAIL mRNA in the decidua tissue of the infected group was slightly higher than that of the non-infected group,but there was no statistically significant difference between the two groups(P>0.05);The expression of DR4 and DR5 mRNA in decidua of infected group was significantly higher than that of non-infected group(P<0.001);On the 6th and 7th day of pregnancy,the expression of DR4 mRNA in the infected rats was negatively correlated with the number of embryos implanted in rats(r=-0.782,P<0.052);The expression of DR5 mRNA in the infected rats was negatively correlated with the number of embryos implanted(r=-0.763,P<0.05);The expression of DcR1 and DcR2 mRNA in the decidua of infected rats was significantly lower than that in the uninfected group(P<0.001).On the 5th,6th and 7th days of pregnancy,there was no significant difference in the expression of TRAIL protein in the decidua of pregnant rats compared with uninfected rats(P>0.05);The expression of DR4and DR5 protein in the decidua of the infected rats was significantly higher than that of the uninfected group(P<0.001);The expression of DcR1 and DcR2 protein in the decidua of the infected rats was significantly decreased compared with the uninfected group(P<0.001).On the 5th,6th and 7th days of pregnancy,the expression of pro-caspase-3 in the decidua of pregnant rats was not significantly different from that of the uninfected group;On the 5th and 6th days of pregnancy,the expression level of active Cleaved-Caspase-3 protein in the infected group was significantly higher than that in the uninfected group(P<0.001).Conclusion:Chlamydia trachomatis infection changed the expression of TRAIL receptors in the decidua of pregnant rats,therefore,it is speculated that Chlamydia trachomatis infection can induce early pregnancy abortion in rats.