The Study Of Action Mechanism On Treating Hyperlipemia Mice With Added Herbs' QingLin Pill

Objective: Added Herbs'QingLin Pill (composed of jiuzhidahuang, dangshen, zhishouwu) has the function of benefiting vital energy, removing stasis and phlegm and is an effective formula through years of clinical observation by Professor YANG Ji-jun. And it has been proved significantly effect in treating hyperlipemia. In order to further probe its action mechanism, we duplicated the hyperlipemia animal model with high-fat forage, At the same time, we interfered the animal with medicine. And we studied the action mechanism of this formula in regulating lipid metabolism through observing its effect on TC,TG,HDL-C,LDL-C; We also studied its action mechanism in resisting lipid peroxidation injury through observing its effect on MDA,SOD; We studied the action mechanism of this formula in vasomotion state and blood flow through observing its effect on ET-1,CGRP,TXB₂ and 6-Keto-PGF_1α.Methods:Partâ… Effect of Added Herbs'QingLin Pill on lipid metabolism of hyperlipemia mice50 healthy male KM mice were divided into 5 groups at random, namely, (1) Normal control group(normal group for short); (2) Model control group(model group for short); (3) Low-dose group of Added Herbs'Qing-Lin Pill group(low-dose group for short); (4) High-dose group of Added Herbs'Qing-Lin Pill group (high-dose group); (5) Simvastatin control group (simvastatin group for short). Except for normal group was fed with ordinary forage, the rest of groups were all fed with high-fat forage. At the same time, the treatment groups were given low-dose of Added Herbs'Qing-Lin Pill 1.5 g /Kg, high-dose of Added Herbs'Qing-Lin Pill 3.0 g /Kg and Simvastatin 6.6 mg /Kg per day. Normal and model group were given distilled water. Mice in each group were given stomach-perfusion once a day and every time the drug volume was calculated by 0.2 ml/10g. Stomach-perfusion lasted for 4 weeks. Then 12 hours after fasting and not prohibiting water of the last time, the mice were removed eyeball and their blood were drawn out, the blood sample was made centrifugation with low temperature and the blood serum was isolated. It was sealed up and keep temperature of under -20℃to examine them later. Finally the contents of TC,TG,HDL-C,LDL-C were measured with biochemistry method. The contents of each item were measured strictly according to the directions of reagent box.Partâ…¡Effect of Added Herbs'QingLin Pill on the content of MDA and the activity of SOD in serum of hyperlipemia miceThe group distribution, duplication of the hyperlipemia animal model and drug-given were exactly same as above. After 4 weeks continuous stomach-perfusion, then 12 hours after fasting and not prohibiting water of the last time, the mice were removed eyeball and their blood were drawn out. The blood sample was made centrifugation with low temperature and the blood serum was isolated. It was sealed up and keep temperature of under -20℃to examine them later. The content of MDA was examined by the method of TBA colorimeter analysis and activity of SOD was examined by the method of xanthine oxidase. The content of each item was measured strictly according to the directions of reagent box.Partâ…¢Effect of Added Herbs'QingLin Pill on the contents of ET-1, CGRP, TXB₂ and 6-Keto-PGF_1α in blood plasma of hyperlipemia miceThe group distribution, duplication of the hyperlipemia animal model and drug-given are exactly same as above. After 4 weeks continuous stomach-perfusion, then 12 hours after fasting of the last time, the mice were removed eyeball and their blood were drawn out. The blood sample was divided two portions and builded in sample tubes of containing different decoagulant and misced bene. The blood sample was made centrifugation with low temperature and the plasma was isolated. It was sealed up and keep temperature of under -80℃to examine them later.Finally the contents of ET-1, CGRP, TXB₂ and 6-Keto-PGF_1α were all measured with radio-immuno method. The content of each item was measured strictly according to the directions of reagent box.Results: Partâ… Effect of Added Herbs'QingLin Pill on lipid metabolism of hyperlipemia miceEffect of Added Herbs'QingLin Pill on the contents of TC, TG, HDL-C and LDL-C in serum of hyperlipemia miceThe contents of TC, TG, LDL-C in serum of model group (5.06±0.90, 1.98±0.41, 2.31±0.39) were all higher than normal group (3.71±0.30, 0.89±0.27, 1.57±0.18) (P < 0.01), while HDL-C of model group (1.43±0.18) was lower than normal group (1.81±0.14) (P<0.01); After the intervention of medicine, the contents of TC, TG, LDL-C in serum of each treatment group were all lower than the model group, while HDL-C was higher than the model group (P<0.05 or P<0.01); The contents of TC, LDL-C and HDL-C in serum were no significant difference in high-dose group (3.95±0.41, 1.78±0.12, 1.69±0.14), low-dose group (3.93±0.30, 1.79±0.13, 1.67±0.15) and simvastatin group (4.02±0.33, 1.82±0.14, 1.66±0.10) (P>0.05). But the contents of TG in low-dose group (1.26±0.45) and high-dose group (1.28±0.19) were lower than simvastatin group (1.61±0.16) (P<0.05). And there was no significant difference between low-dose and high-dose group (P>0.05).Partâ…¡Effect of Added Herbs'QingLin Pill on the content of MDA and the activity of SOD in serum of hyperlipemia mice1 Effect of Added Herbs'QingLin Pill on the content of MDA in serum of hyperlipemia miceThe content of MDA in serum of model group (20.94±3.49) was higher than the normal group (11.94±1.44) (P<0.01); After the intervention of medicine, the content of MDA in serum of each treatment group was lower than the model group (P<0.01); But the contents of MDA in low-dose group (15.87±1.51) and high-dose group (13.77±1.41) were lower than simvastatin group (17.95±1.24) (P<0.05 or P<0.01). The high-dose group was lower than the low-dose group (P<0.05).2 Effect of Added Herbs'QingLin Pill on the activity of SOD in serum of hyperlipemia miceThe activity of SOD in serum of model group (160.6±10.64) was lower than the normal group (236.2±12.89) (P<0.01); After the intervention of medicine, the activity of SOD in serum of each treatment group was higher than the model group (P<0.01); But the activity of SOD in low-dose group (193.0±11.55) and high-dose group (205.7±11.82) were higher than simvastatin group (181.9±11.40) (P<0.05 or P<0.01). The high-dose group was higher than the low-dose group (P<0.05).Partâ…¢Effect of Added Herbs'QingLin Pill on the contents of ET-1, CGRP,TXB₂ and 6-Keto-PGF_1α in blood plasma of hyperlipemia mice1 Effect of Added Herbs'QingLin Pill on the content of ET-1 in plasma of hyperlipemia miceThe content of ET-1 in plasma of model group (126.95±15.46) was higher than the normal group (67.78±7.04) (P<0.01); After the intervention of medicine, the content of ET-1 in plasma of each treatment group was lower than the model group (P<0.01); But the contents of ET-1 in low-dose group (85.41±9.93) and high-dose group (74.29±6.65) were lower than simvastatin group (100.43±9.44) (P<0.01). The high-dose group was lower than the low-dose group (P<0.05).2 Effect of Added Herbs'QingLin Pill on the content of CGRP in plasma of hyperlipemia miceThe content of CGRP in plasma of model group (54.83±7.18) was lower than the normal group (105.65±11.65) (P<0.01); After the intervention of medicine, the content of CGRP in plasma of each treatment group was higher than the model group (P<0.01); But the contents of CGRP in low-dose group (80.49±8.28) and high-dose group (89.63±8.18) were higher than simvastatin group (67.54±6.79) (P<0.01). The high-dose group was higher than the low-dose group (P<0.05).3 Effect of Added Herbs'QingLin Pill on the content of TXB₂ in plasma of hyperlipemia miceThe content of TXB₂ in plasma of model group (2704.24±370.59) was higher than the normal group (1514.64±342.93) (P<0.01); After the intervention of medicine, the content of TXB₂ in plasma of each treatment group was lower than the model group (P<0.05 or P<0.01); But the contents of TXB₂ in low-dose group (1893.38±237.39) and high-dose group (1883.46±332.30) were lower than simvastatin group (2294.48±340.49) (P<0.05). And there was no significant difference between low-dose and high-dose group(P>0.05).4 Effect of Added Herbs'QingLin Pill on the content of 6-Keto-PGF_1α in plasma of hyperlipemia mice The content of 6-Keto-PGF_1α in plasma of model group (924.37±153.28) was lower than the normal group (1370.19±131.21) (P<0.01); After the intervention of medicine, the content of 6-Keto-PGF_1α in plasma of each treatment group was higher than the model group (P<0.05 or P<0.01); But the contents of 6-Keto-PGF_1α in low-dose group (1245.92±157.26) and high-dose group (1236.70±144.09) were higher than simvastatin group (1093.73±135.86) (P<0.05). And there was no significant difference between low-dose and high-dose group (P>0.05).5 Effect of Added Herbs'QingLin Pill on TXB₂/ 6-Keto -PGF_1α in plasma of hyperlipemia miceThe TXB₂/6-Keto-PGF_1α in plasma of model group (2.98±0.52) was higher than the normal group (1.10±0.23) (P<0.01); After the intervention of medicine, the TXB₂/6-Keto-PGF_1α in plasma of each treatment group was lower than the model group (P<0.01); But the TXB₂/6-Keto-PGF_1α in low-dose group (1.47±0.24) and high-dose group (1.53±0.31) were lower than simvastatin group (2.12±0.40) (P<0.05). And there was no significant difference between low-dose and high-dose group (P>0.05).Conclusion:1 Added Herbs'QingLin Pill has decreased the contents of TC,TG,LDL-C and increased the content of HDL-C in serum. The result shows that it has marked function of regulating lipid metabolism. 2 It obviously increased the activity of SOD, and decreased the content of MDA in serum. The result shows that it has marked function of inhibiting lipid peroxidation reaction and raising anti-oxidative ability. It could reduce oxidative damage of hyperlipemia through amending free radical metabolism.3 It obviously decreased the contents of ET-1 with TXB₂ and increases the contents of CGRP with 6-Keto-PGF_1α in plasma. And it obviously regulated the proportion of TXB₂ and 6-Keto-PGF_1α in plasma. The results shows that it has marked function of regulating the normal contraction and dilation state of vascular smooth muscle , which in order to remove the platelet coagulation with thrombosis. It could prevent the blood vessel endothelium damage of hyperlipemia and protect blood vessel endothelium function.