Experimental Research About The Variation Of Immune Function In UC And The Intervention Of Qi Xian An Chang Recipe

Objective: We also found out the main disease mechanism of UC was Pi weakness, chronic accumulation of Tanshi and further turning Shire.Qi Xian An Chang Recipe is a effective therapy, so we made up a therapy principle of healthiness spleen, dispelling damp and disappeaering carbuncle.Through the research and experiment, we could observe Qi Xian An Chang Recipe's effects on UC and demonstrate its the immune functional mechanism.Methods: The animal experiment copied a rat UC model induced by rinitrobenzene-sulfonic acid(TNBS) and alcohol.The day after the model being made(MK), we cured them with DaJiLiang group (DK), ZhongJiLiang group(ZK), XiaoJiLiang group(Xk) and LiuDanHuangAnBiDing group(LK), observing their effects.The experiment contained four components.Experiment I: was to observe the the general state of UC rat, their pathological changes and the RBC immune;Experiment II: was about Qi Xian An Chang Recipe's effect on T cell subsetand the immune globulin and the complement.Experiment III was about Qi Xian An Chang Recipe's effect on the expression of ICAM-1 protein.Through these experiments we could explore UC disease mechanism further.Results:1 Experment I:1.1 The general condition (DAI)Compared with MG(2.016±0.401), DK(0.429±0.153), ZK (0.496±0.144), XK(1.304±0.212), LK(1.286±0.299)and BK (0.378±0.133)all could significant decrease the General state in UC rat (P<0.01); LK was compared with three Chinese herbal groups: DK, ZK was significant better than LK (PDK-LK<0.01) (PZK-LK<0.01); Compared among the three Chinese herbal: DK, ZK was significant better than XK (PDK-XK<0.01) (PZK-XK<0.01).1.2 Pathological damageMG rats'colonic mucosas were unabroken, with congestion, edema and infiltrating of inflammatory cells.LK and three Chinese herbal groups'colonics mucosas were unabroken, without such appearance above-mentioned.1.3 The RBC immuneRC3bR: Compared with MG(4.50±1.75), DK(8.5±1.58), ZK (8.31±1.75) and BK(9.31±2.09)all could significant increase the content of RC3bR(P<0.01);LK(6.49±2.41)was compared with three Chinese herbal groups: DK was significant increase the content of RC3bR(PDK-LK<0.05);Compared among the three Chinese herbal: DK was significant better than XK(6.46±2.18) (PDK-XK<0.05).RICR: Compared with MG(2.625±0.916), DK(0.875±0.732), ZK(0.938±0.729) and BK(0.813±0.651)all could significant decrease the content of RCIC (P<0.01);LK(1.846±0.620)was compared with three Chinese herbal groups: DK, ZK was significant decrease the content of RCIC (PDK-LK<0.05)(PZK-LK<0.05);Compared among the three Chinese herbal: DK, ZK was significant better than XK(1.825±0.667) (PDK-XK<0.05)(PZK-XK<0.05).2 Experimen II:2.1The T cell subset(%)CD3:Compared with MG(65.98±2.54),DK(52.48±4.35), ZK(53.52±3.73) and BK(48.98±3.48)all could significant decrease the percentage of CD3+(P<0.01);LK(61.09±1.42) was compared with three Chinese herbal groups: DK, ZK was significant decrease the percentage of CD3 + (PDK-LK<0.01) (PZK-LK<0.01); Compared among the three Chinese herbal: DK, ZK was significant better than XK(61.33±8.53)(PDK-XK<0.01) (PZK-XK<0.01).CD4+: Compared with MG(51.03±3.37), DK(25.24±4.40), ZK(26.49±3.07), XK(39.97±4.48), LK(40.36±4.63)and BK (25.18±4.42)all could significant decrease the percentage of CD4+ (P<0.01);LK was compared with three Chinese herbal groups: DK, ZK was significant decrease the percentage of CD4+ (PDK-LK<0.01)(PZK-LK<0.01); Compared among the three Chinese herbal: DK, ZK was significant better than XK (PDK-XK<0.01)(PZK-XK<0.01).CD8+: Compared with MG (14.69±3.45), DK(27.80±7.29), ZK(27.78±6.96)and BK(27.65±6.80)all could significant increase the percentage of CD8+ (P<0.01);LK(21.22±6.27)was compared with three Chinese herbal groups: DK, ZK was significant increase the percentage of CD8 + (PDK-LK<0.01) (PZK-LK<0.01);Compared among the three Chinese herbal: DK, ZK was significant better than XK(21.28±6.34) (PDK-XK<0.05) (PZK-XK<0.05).CD4+/CD8+: Compared with MG(3.469±0.224), DK(1.008±0.254), ZK(1.034±0.245), XK(1.869±0.043), LK(1.868±0.129) and BK(0.991±0.191)all could significant decrease the percentage of CD4+/CD8+ (P<0.01); LK was compared with three Chinese herbal groups: DK, ZK was significant decrease the percentage of CD4+/CD8+(PDK-LK<0.01) (PZK-LK<0.01); Compared among the three Chinese herbal: DK, ZK was significant better than XK(PDK-XK<0.01)(PZK-XK<0.01).2.2The immune globulinIgA: Compared with MG(0.088±0.024), DK(0.036±0.017), ZK(0.041±0.019)and BK(0.035±0.022)all could significant decrease the content of IgA (P<0.01);LK(0.063±0.026)was compared with three Chinese herbal groups: DK, ZK was significant decrease the content of IgA(PDK-LK<0.05) (PZK-LK<0.05);Compared among the three Chinese herbal: DK, ZK was significant better than XK(0.064±0.025) (PDK-XK<0.05) (PZK-XK<0.05).IgG: Compared with MG(0.169±0.082), DK(0.204±0.068), ZK(0.210±0.085)and BK(0.169±0.082)all could significant decrease the content of IgG (P<0.01);LK(0.300±0.105)was compared with three Chinese herbal groups: DK, ZK was significant decrease the content of IgG(PDK-LK<0.05) (PZK-LK<0.05);Compared among the three Chinese herbal: DK, ZK was significant better than XK(0.300±0.100)(PDK-XK<0.05) (PZK-XK<0.05).IgM: Compared with MG(0.309±0.070), BK(0.195±0.066) Could significant decrease the content of IgM (P< 0.01);Compa red among the others: DK(0.271±0.056), ZK(0.272±0.050), XK (0.297±0.041), LK(0.300±0.050)were not significant the variation( P>0.05).2.3The complement C3,C4C3: Compared with MG(0.130±0.037), DK(0.209±0.038), ZK(0.206±0.018)and BK(0.216±0.036)all could significant increase the content of C3 (P<0.01);LK(0.171±0.030)was compared with three Chinese herbal groups: DK, ZK was significant increase the content of C3(PDK-LK<0.05) (PZK-LK<0.05);Compared among the three Chinese herbal: DK, ZK was significant better than XK(0.169±0.030) (PDK-XK<0.05) (PZK-XK<0.05).C4: Compared with MG(0.024±0.011), DK(0.058±0.016), ZK(0.050±0.011)and BK(0.059±0.019)all could significant increase the content of C4 (P<0.01);LK(0.040±0.016)was compared with three Chinese herbal groups: DK was significant increase the content of C4(PDK-LK<0.05);Compared among the three Chinese herbal: DK was significant better than XK(0.040±0.019) (PDK-XK<0.05). 3 Experiment III: The expression of the Intercelluar adhesion molecule-1Compared with MG(5.750±1.282), DK(1.875±0.991), ZK (2.125±0.991), XK(2.625±0.916), LK(2.750±1.165) and BK (1.125±0.991)all could significant decrease the expression of the Intercelluar adhesion molecule-1 (P<0.01);Compared among the others were not significant the variation.( P>0.05).Conclusion:Qi Xian An Chang Recipe can improve UC rat's general condition and colonic mucosa pathological damage;increase the content of C3, C4lessen the content of IgG, IgA in blood serum and decrease the expression of the Intercelluar adhesion molecule-1, IgM hadn't significant variation between treating before and after.Qi Xian An Chang Recipe can improve UC rat's the content of RC3bR in blood serum and decrease the content of RCIC in blood serum to lessen colonic mucosa damage;this Recipe also adjust the balanced between CD4+ and CD8+ and the disbalanced between CD4+and CD8+will cause immune hyperfunction.especially DK, ZK's integrity effect is the best, significantly better than others.