The Experimental Study On Music Electro-Accupuncture Adjustment Zymoplasm Level Of Acute Cerebral Hemorrhage Mouse To Protect Of Lesions Of Around Neurons

Objective:In discussion music pulse electric acupuncture(EA)adjustment brain zymoplasm to neuron cell influenceMethod:The rats were randomly divided into control group,model group,pulse EA group,music pulse EA group.Uses the collagenase to add the heparin to induce the mouse caudate putamen cerebral hemorrhage the animal model.Observes 6h,24h, 3d,the 7d different time mouse brain water content,the PAR-1 expression level,the percentage of the neuron apoptosis cell.Using does the wet weight law to determine the mouse brain water content;The immunohistochemical method examines PAR-1 the expression level,flow cytometer examination the percentage of the neuron cell apoptosis,the TUNEL law examination the shape of neuron cell apoptosis.This experiment take the purebred male Wistar mouse(250-300 gram)as the experimental animal duplication acute cerebral hemorrhage model in caudate putamen of brain. Uses music pulse EA and the pulseEA carries on the treatment of the cerebral hemorrhage big mouse and makes the contrast observation to its curative effect,after cerebral hemorrhage different time we take the shape of the neuron apoptosis cell, the percentage of the neuron apoptosis cell,the zymoplasm,the brain organization water content as the observation target to compartment brain neuron cell and the organization dropsy change in the haematoma central area and carries on the relevant analysis.From molecular level angle research The blood contents zymoplasm and apoptosis cell after cerebral hemorrhage.Confirmed music pulse EA affects for the hydro-cephalus and adjusts the zymoplasm level related after the acute cerebral hemorrhage.Discused music pulse EA treat acute cerebral hemorrhage mechanism.Results:Music electro-acupuncture group at a number of electro-acupuncture treatment,in rat brain PAR-1 protein expression was significantly weakened,neuron apoptosis decreased significantly,and electro-acupuncture group compared to a significant difference. #属性不符...