Influence Of Interleukin-18 Of Human Aqueous In Diabetic Retinopathy

Objectives: 1. To measure the interleukin-18(IL-18) levels in aqueous with type 2 diabetes mellitus(T2DM) and diabetic retinopathy(DR), and approach the effects of IL-18 on the occurrence of DR.2. To measure the IL-18 levels in aqueous with non-proliferative diabetic retinopathy(NPDR) and proliferative diabetic retinopathy(PDR) respectively,and approach the effects of IL-18 on the development of DR.3. To estimate the relation between inflammatory factors and diabetic retinopathy(DR),investigate the mechanism of DR and provide theoretical basis for the prevention and treatment of DR.Methods: The cases came from inhospital patients of the first affiliated hospital of Chongqing Medical University.All the Patients were divided into 4 groups according to the fol1owing standards.Group-A (normal control group): 10 cases of health adults who have cataract,without diabetes mellitus,hypertension,nephrosis or any other ophthalmocaces.Group-B (diabetic group): 10 cases of T2DM without diabetic retinopathy.Guoup-C (NPDR group): 10 cases of diabetes with NPDR in hospital.Guoup-D (PDR group): 10 cases of diabetes with PDR in hospital. Enzyme linked immunosorbent assay(ELISA) was used for detecting aqueous IL-18 levels.All the datas were inputed into computer and analyzed with SPSS for windows 13.0 software.Results: 1.No significance differences were found among all the groups in age and male-female ratio.2. The fasting blood glucose was similar for the each subgroup(P>0 .05).3. IL-18 concentrations in diabetics were significantly higher than those in controls(126.20±6.04 pg/ml for controls,n=10;P<0.001).IL-18 concentrations in patients with proliferative diabetic retinopathy (n=10,(3.34±1.06)×103pg/ml) and non-proliferative diabetic retinopathy (n =10,(1.42±0.14)×103pg/ml) were significantly higher than those without diabetic retinopathy (n=10,182.37±38.33 pg/ml;P<0.01).4. The aqucous IL-18 levels were singnificantly different among the groups(P<0.01) and increased gradually in groups.IL-18 levels in group D patients were all higher than 2000pg/ml.Conclusion: Hyper-interleukin-18 in aqueous may participate in development and pathogenesis of DR. IL-18 plays an important role in neovascularization of diabetic retinopathy.