The Effect Of Different Methods Of Hypoxia Training On The Cellular Of Mice Skeletal Muscle And Relative Biochemicalindexes

Simulated hypoxic training is a new scientific training method, which plays an active role in training. This experiment, which make use of hypoxic cabin equipment simulating different hypoxic training method, building-up animal training method, applied theory and method of morphology, athletic physiology and sports biochemistry statistical to analyze rats' skeletal muscle ultrastructure, and bio-chemical metabolism index. Investigation and discussion which is from the influence of the different hypoxic method upon effect of the rats' skeletal muscle ultrastructure, bio-chemical metabolism looking for under hypoxic circumstance carried on the best training method of the plateau training.Experimental methods:Experiment is with the male healthy SD rats for test object, building up the low oxygen and normal oxygen of rat to train model,at experiment's request ,which is randomly divided into: sea level sedentary group(C), Living low Training low (LoLo), sedentary group of hypoxia(HC), Living low Training High (LoHi), Living High Training low (HiLo), Living High Training High (HiHi), Living High Training High Training low (HiHiLo). The sport set carries on gradually intensive pedestal training once a day,at 08:00-11:00,with the speed from 20m/min to 30m/min and time from 30min to 60min.After four weeks',the rats were killed by decollation after exhaustive running,and changes of in weight,Hb,Hct,the blood sugar,BLa,and the ultrastructure of skeletal muscle of rats,SOD,MDA,MLa,CK,MDH, ATPase index sign of the skeletal muscular tissue are tested.Results of the experiment:1. After hypoxia training, HiLo and HiHiLo show fine adaptation in skeletal muscle ultrastructure, for example, Marshalling of muscle fibre, an increase of chondrioso -me,and the entire bilayer film ,the compact ridge .But LoHi group of skeletal muscle loss is relatively bigger grave.At the same time,the three sets of rats of skeletal muscle glycogens contents are notably increased.2. Hb and Hct contents of the hypoxia group are obviously higher than the normal oxygen group after hypoxia training.But the different pattern improves differently, HiLo and HiHiLo group are better the that other group. It indicats that the hypoxic training can strengthen the oxygen carrying capacity. At the same time,the blood sugercontents of HiLo and HiHiLo group are higher notably than other, it can also promotethat aerobic exercise performance is more helpful.3. Skeletal muscle MDA content of the hypoxia group rats descend remarkably andcorresponding SOD activity go up notably after hypoxia training. HiLo,HiHiLo groupare better than LoHi group.It indicats that the hypoxic training can improve the rat'skeletal muscle oxidation resistance.4.In hypoxia training group, the rats MLa and BLa contents descend remarkly indifferent degree. And HiLo group decreased obviously compared with LoHi, HiLoand HiHiLo. It indicats that the hypoxic training reduce lactic acid contents andprotect them by improving their aerobic-metablizability.5.The rat's Skeletal muscle MDH activity of HiLo group is higher than others afterhypoxia training.And CK activity of LoHi,HiLo and HiHiLo group are higherobviously than LoLo and HiHi group. It indicats that the hypoxic training canstrengthen the rat's keletal muscle oxidase activity and CK activity, promotingaerobic metabolism ability have very big help.6. The rat's Skeletal muscle Na⁺,K⁺-ATPase activity of HiHiLo and HiLo group ishigher than the others after hypoxia training,and skeletal muscle Mg²⁺ -ATPaseactivity of HiLo group is also higher than LoHi and HiHiLo group. It indicats that thehypoxic training can improve ATPase activity.conclusion:1.After hypoxia training, HiLo and HiHiLo show fine adaptation in skeletal muscleultrastructure.But Skeletal muscle cells of LoHi group represent anoxia damage inaspect of muscle fiber, mitochondria and capillary vessel in some degree. It indicatsthat the rat's Skeletal muscle cells produce different influence because of differenthypoxic training.2.The different hypoxia training methods make different effect in aspect of the rats'skeletal muscle bio-chemical index, it can promote aerobic metabolism ability andsports ability in different degree. Of all these kinds of hypoxia training methods,HiLo shows a better result than the other, and followed by the HiLo.