Expression Of Platelet Derived Growth Factor Receptor In Gluteal Muscle Contracture

[Objective] To explore effect of Platelet derived growth factor on gluteal muscle contracture by investigating the expression of Platelet derived growth factor receptor in Gluteal Muscle Contracture patients distinct tissues. And provide a theoretical basis for the prevention and clinical treatment of gluteal muscle contracture.[Methods] The samples obtained via operation from 20 patients with Gluteals Muscle Contracture (GMC), and Harvested by adjacent normal muscle from GMC 20 patients, the other with 20 muscle tissue samples from normal children as the control. To observe the pathological changes with HE staining and the immune expression of PDGF-R using immunohistochemistry in all groups. Furthermore, count the mean observed density (MOD) of all the samples. At last, all the data were conducted with SPSS 13.0 statistical software.[Results] 1. The results of H.E staining show us:(1) Normal group:Muscle cells were fibrous, and non-branching on the longitudinal section. There are obvious cross striations, numbers of nucleus which showed flat oval. Nucleus is located beneath the cell membrane. There are a number of filamentous parallel myofibril along muscle cells axis. From cross-section observations show that muscle cells are red muscle cells of polygons of varying sizes. Myofibril was red dotted. Myofibrils arranged evenly in the muscle cytoplasm, the oval nucleus is located cells around in larger quantities。(2) Pathological changes group:Contracture of gluteus muscle cells can be seen atrophy, degeneration, mostly focal, but there are remnants of the ultra structure of skeletal muscle fibers. It has lost the normal skeletal muscle structure. The boundaries with the normal muscle fibers is unclear, intertwined. Muscle cell cross stripes disappeared, nucleus concentration, deep dyeing, fragmentation, dissolution or even absence, collagen fibers increased, Some local and even showed hyalinization, And the formation of unstructured homogeneous material. The blood vessels of inter-muscular are decreased in the number, and vessel wall is thickened, the lumen of small and not rules have occlusion, infiltration of neutrophils and lymphocytes can be seen in vascular. Fibrosis region in connective tissue has large areas of dense collagen fibers (maybe need to change); and the process of evolution from striated organizational structure of degeneration to the fibrous tissue can be seen in some regions. Some cases can be seen proliferation of fibrous tissue in the interstitial as well as a small amount of inflammatory cell infiltration, moreover, macrophages. (3) Lesions adjacent tissue group:With a normal skeletal muscle structure, we found the light band and the dark band are basically in one plane in the adjacent myofibrils, The phenomenon of both the infiltration of inflammatory cells and the collagen fibers hyperplasia have already been seen in the muscle cell. The invasion of inflammatory cells clearly in some samples demonstrated the characteristics of chronic inflammation via lymphocytes, plasma cells and mononuclear cell infiltration. The muscle fibers are also visible focal swelling, degeneration, muscle rupture, or even destruction of muscle cells, arranged in disorder, muscle cells, vary in size, muscle gap widened, but also see the formation of granulation. Generally speaking, most of the muscle fibers were arranged in order and no reduction of blood vessels in the inter-muscle. But some shows vascular lumen smaller, wall thickening.2. Immunohistochemical Observation:PDGF receptor in the three groups of positive staining cells were fibroblasts, small blood vessel endothelial cells and vascular smooth muscle cells, monocyte-macrophage cells, muscle cells, fibroblasts, inflammatory cells. The results of staining intensity showed a weak positive in the most of the normal group and enhanced significantly in pathological changes group and Lesions adjacent normal tissue group; meanwhile, positive signals were mainly located in cytoplasm membrane, Blank control without brown coloring. Positive cells of PDGF receptor expression is the largest number in lesions, shows a large number of fibroblasts, which are diffuse distribution, Darker color, arranged disorders, the yellow particles of positive expression are mainly distributed in the membrane and the cytoplasm of fibroblasts. The other cells can also be found positive staining. The immune target is the cytoplasm membrane in all positive cells, and are measured the average optical density value respectively. Statistical method is used comparison among the three groups by analysis of variance. Among the three groups there was significant difference each other. For lesions adjacent tissue group, we have compared between a long course of the disease group and the short duration group. Significant difference was found between the two groups.[Conclusions] 1. PDGF receptor expression of groups shows that PDGF receptors are involved in the pathogenesis of the GMC.2. The elevated levels of PDGFR might facilitate their responses to PDGF in GMC fibroblasts, which seem to play an important role in the formation of GMC. And the continued presence of PDGF in patients may be an important factor for the increased fibrosis in GMC patients.