| ObjectiveObesity as a global metabolic syndrome disease, it can induce a variety of diseases such as diabetes, hypertension, coronary heart disease and so on, which are seriously affect the human health. Studies have shown that zinc-a2-glycoprotein ZAG (Zn-a2-glyco protein, ZAG) is a lipid mobilizing factor, plays an important role in promoting the breakdown of adipose tissue, and body weight reducing. The aim of this study is to observe the effect of aerobic exercise on the expression of ZAG in adipose tissue of obese rats, to explore the mechanism of aerobic exercise on improving glycolipid metabolism, so as to provide a new theoretical basis and ideas for the treatment of obesity.MethodSixty-six male SD rats were randomly divided into control group (C, n=16) and high fat group (H, n=50). The rats in the C group were fed with a standard diet, and the rats in the H group were fed with a high fat diet. At the end of the 7th week, the obese rats (DIO, n=24) which body weights were more than 20 percent of the C group's from the H Group were randomly divided into three groups:obese control group (N, n=8), obese exercise group (E, n=8) and the death group (n=8). The rats in each group were fed with a standard diet. In the following 6 weeks, the rats in E group were given a daily treadmill exercise (8-18m/min,0% grade,60 min/day,6 days/week). At the end of the 7th and 13th week, the body weight, visceral fat weight, relative visceral fat weight, FBG, FINS, ISI, TG were measured and analyzed in each group. And the expression of PPARĪ³in the soleus muscle was detected with fluorescence quantitative polymerase chain reaction (PCR).Result1. Body weight:There were no significant difference between the C group and the H group before experiment (P>0.05). At the end of the 7th week, the DIO group's body weight (P<0.01) was significantly higher than the C group's. There were no significant difference between the N group and the E group (P>0.05), and both of the groups' body weight (P<0.01) were significantly higher than the C group's before exercise. At the end of the 13th week, there were no significant difference among the three groups (P>0.05).2. Visceral fat weight and relative visceral fat weight:At the end of the 7th week, the DIO group's were significantly higher than the C group's (P<0.01). At the end of the 13th week, the N group's were significantly higher than the C group's (P<0.05) and the E group's (P<0.01). The E group's were lower than the C group's (P>0.05).3. Blood biochemical indicators: (1) Fasting blood glucose (FGB)FBG after modeling obese group was significantly higher (P<0.01); exercise before the intervention, obese group and obese exercise group quiet FBG showed no significant difference(P>0.05), were significantly higher (P<0.01). After the intervention, obese exercise group FBG levels lower than the control group (P<0.05) and obesity quiet group (P<0.05); obesity quiet group and the control group no significant difference (P> 0.05)(2) Fasting insulin (FINS)FINS and the obese group after modeling with the control group no significant difference. Exercise before the intervention, obese FINS quiet group was significantly higher than obese exercise group (P<0.01) and the control group (P <0.01). Exercise intervention, obese exercise group and control group no significant difference (P>0.05).(3) Insulin sensitivity index (ISI)Modeling, control group and compared between the obese group there was no significant difference. Exercise intervention, the three group ISI was no significant difference(P>0.05), but the obese group and obese quiet exercise group than the control group decreased. Exercise intervention, obesity ISI quiet group than control group (P<0.05) and obese exercise group (P<0.01), obese exercise group than the control group tended to increase(P>0.05).(4) Serum triglyceride (TG)Model, the obese group higher serum TG levels (P<0.05). Exercise before the intervention, obese control group exercise group, the difference was statistically significant (P<0.05). After the intervention, obesity was significantly higher than the quiet group (P<0.01) and obese exercise group (P<0.01), the control group and obese exercise group compared with no significant difference (P> 0.05)4. Rat adipocytes ZAG mRNA expression levels:exercise intervention after the exercise group obese rat adipocytes ZAG mRNA expression levels were significantly higher than obese quiet group (P<0.01), compared with the control group, no significant difference, but tended to increase significantly, and both were significantly higher than the quiet group.Conclusions1. Aerobic exercise can increase insulin sensitivity and the expression of ZAG gene in the adipose tissue of obese rat.2. ZAG expression in obese rats with body weight, fat pad weight, FGB, FINS, and TG levels were negatively correlated, positively correlated with the ISI, indicating ZAG expression may promote the body's lipid metabolism.3. ZAG mRNA expression and fat pad weight, relative weight and insulin were negatively correlated with insulin sensitivity.Being index is positively correlated with body weight had no correlation, indicating that ZAG expression in that the obese body weight loss was mainly with the fat content down, but not with no cause lean body mass decreased significantly, which for the weight loss therapy new targets, especially for athletes before the application of weight reduction prospects good. |
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