The Orange Six Herbs As Well As Drug Of Ginger - Citrus Aurantium, Honeysuckle - Forsythia Chemical Composition

Traditional Chinese Medicine (TCM) is an analytical system with too much complexity. Usually, there are a lot of chemical components in single herbs and most of them are with low concentration. As a result, commonly analytical instruments and detecting approaches can not make it possible to qualitatively and quantitatively determine chemical components from single herbs completely. With the advent of hyphenated chromatographic instruments, such as GC-MS and HPLC-DAD, and the rapid development of some chemometric multi-resolution techniques like Heuristic Evolving Latent Projections (HELP), it is possible to completely conduct the qualitative and quantitative analysis for TCM by use of the combined information on separation and identification produced by chromatography and spectrometry.The main aim of this thesis is to systematically investigate the volatile chemical components of several herb pairs (HPs) and the Citrus reticulata Blanco by hyphenated instruments combined with chemometric resolution method, and then carrying out qualitative and quantitative analysis for TCM. In total, this thesis covers three parts as below.1. Qualitative and quantitative analysis of the volatile chemical components in HP Zingiber officinale Rosc (ZOR)-Fructus aurantii Immaturus (FAI) with hyphenated GC-MS and CRM (chapter 2). The volatile oils from ZOR, FAI and ZOR-FAI were respectively extracted, and were separated and detected by GC-MS. The two-dimensional GC-MS data were resolved and processed by the HELP method to obtain pure chromatographic curves and MS profiles, and then the volatile components were identified. Therefore, the reliability and accuracy of qualitative analysis were greatly enhanced because the results are obtained from the pure mass spectrum of each compound. Identification of the compounds was also assisted by comparison of temperature-programmed retention indices (PTRIs).2. Systematical investigation of the volatile components in HP Flos lonicerae (FL)-Fructus forsythiae (FF) (chapter 3). The volatile components from the HP FL-FF are pharmaceutically active. And thevolatile components from FL, FF and HP FL-FF were separated and detected by GC-MS, and the comparative analysis of common volatile constituents between the HP FL-FF and its single herbs was performed by alternative moving window factor analysis (AMWFA) that illustrates the changes in volatile components after compatibility. And resolved other overlapped peaks that can not be comparatively analyzed by AMWFA, then implemented a comprehensive and systematic analysis of the chemical components.3. Studies on the volatile components in Album citri Reticulatae(ACR), Cylindricae citri Reticulatae(CCR), Folium citri Reticulatae(FCR), Exocarpium citri Grandis(ECG), Pericarpium citri Reticulatae Viride(PCRV) and Pericarpium citri Reticulatae(PCR) (chapter 4). The hyphenated GC-MS was used to separate and detect the volatile oils from them, and then AMWFA was employed to comparatively analyze the volatile constituents among them. Excavating the inter-relationship in components to clarify the similarities and differences in the chemical components aspects in the six medicines. And the chemical composition of the six herbs was also comprehensively analyzed with the help of HELP.