Studies On The Development And Mechanisms Of Protease Enzyme Preparation Used In Tabacco

The inherent quality of tobacco is an important basis for determining the tobacco availability, the cured tobacco leaf protein residues will affect cigarette smoking quality, thereby affecting the intrinsic quality of the tobacco. In recent years, a growing number of studies have shown that the use of hydrolytic enzymes produced by microbes can effectively degrade the protein content of tobacco leaves, the intrinsic chemical composition of tobacco leaves tend to coordinate to achieve the purpose of tobacco quality. Therefore, how the effective use of enzyme technology processing tobacco in tobacco macromolecules moderate conversion to improve the intrinsic quality of tobacco, and improve enterprise efficiency, has become an important technology for the tobacco industry concern.In this study, laboratory screening of producing protease strains of Bacillus pumilus (B. pumilus)(B1) on the surface of tobacco leaves fermentation, and preparation of the enzyme preparation. This protease secreted extracellular enzyme. Enzymatic properties that the findings suggest that the protease had good catalytic ability of a variety of protein substrates. When amino acids of the polypeptide main chain a-carbon atoms, even the R group is inconsistent, when the R group is methyl, the highest enzymatic substrate, and the peptide main-chain-containing heterocyclic amino acids, enzymes The highest catalytic ability. The optimum temperature is50℃, the optimum pH was6.0, Optimization of fermentation conditions, the final activity doubled.We found that the amount of enzyme in the conditions of the enzyme preparation to deal with tobacco and tobacco moisture to reduce tobacco protein, to improve the content of amino acids significantly, while the influence of temperature and time was not significant. Enzyme the optimum working conditions:enzyme concentration120U/g, tobacco, tobacco leaf temperature of45℃,30%of the tobacco moisture160U/g, in this conditions, rate of protein degradation isl8.27, rate of amino acids ncrease isl9.71%.Scale amplification experiments, the compound Bl protease preparation to deal with the2011Yuxi K326C4F redried leaf tobacco, The results show that the processing of tobacco leaf protein content are significantly reduced, the amino acid content was increased.enzyme group" sugar alkali "and"" sugar nitrogen "were higher than the control group, the smoking results show that the sensory quality of the treated sample is improved significantly.Therefore, from the use of tobacco leaf surface screening with indigenous microbial strains preparation of enzyme, not only has the excellent safety, at the same time, it has strong pertinence to degrade the protein and other substances in tobacco leaves, and when compared with the existing commercial enzyme treatment effect is good and can generate more and improve the quality of tobacco leaves material...