| The research on effects of sodium selenite either on regulating the breeding ability ofmutton sheep, or on improving serum Se level, meat production and meat quality of castratedkids was conducted to provide some technical basis for a reasonable feeding system of sheepand goats in the selenium-deficient areas.Trial1.24two-year old Suffolk managed under stall-feeding condition were divided intothe treatment and control group randomly. The treatment group was injected with4ml sodiumselenite-vitamin E (Se0.46mg/ml) before superovulation treatment, while the control groupwas injected with nothing. The quality and quantity of embryo of Suffolk sheep wereevaluated during the embryo transfer, the serums were prepared to determine the levels ofP4,E2,FSH,LH,FT3and FT4at the25th-day after treatment. The results showed that8.7available embryos were collected from the treatment group (2.1more than that of the controlgroup). The serum P4, E2concentrations of treatment group were8.46±4.54ng/ml and13.49±4.41pg/ml, respectively, and both of them were significantly higher than that ofcontrol group (P<0.05). The serum FSH and LH levels of treatment group were5.46±1.55and4.12±1.19mIU/ml, showing no notable difference between these two groups. The serum FT3and FT4levels were4.67±0.71and10.23±2.32pmol/L, respectively, with FT4levelsignificantly lower than that of the control group(P<0.05). Hence, the quantity of availableembryo and serum P4and E2of Suffolk sheep used for donor were improved by the injectionwith Sodium selenite-vitamin E.Trial2. A total of255-month castrated Boer goat crossbred kids under stall-feedingcondition were divided into4treatment groups and the control group randomly. Those goatsfrom treatment groups Ⅰ, Ⅱ, Ⅲ and Ⅳ were then injected respectively with sodiumselenite-vitamin E1ml (Se0.46mg),2ml(Se0.92mg),3ml(Se1.38mg) and4ml(Se1.84mg), and then weighed at the0day. The control group was injected with nothing. The serumsof every group were collected at the morning at day0,1,2,3,10,20,30,40and50. Theserums were prepared to determine the concentrations of Cr,BUN,CHO,TG,Glu,T-SOD and Se,3goats were randomly chosen from each group and slaughtered at the end of the trial.The carcass were segregated and samples of the eye muscle area and back-up muscles for thedetermination of crude protein, crude fat, loss of moisture, ash, dripping and selenium content.The results are listed as follows:(1) There were no significant difference, either among those treatment groups or betweentreatment groups and control group on daily gain, carcass weight, slaughter rates, net rates ofmeat, as well as flesh and blood, eye muscle area and GR values (P>0.05).(2) The serum Se levels of treatment groups were significantly higher than that of thecontrol group (P<0.05), The Sequence of serum Se levels was treatment Ⅳ(198.85±10.72μg/L)>treatment Ⅲ (188.26±6.79μg/L)>treatment Ⅱ(159.88±5.19bμg/L)>treatmentⅠ(111.74±8.57μg/L)>control (22.71±4.47μg/L). The levels oftreatment groupⅡ,Ⅲand Ⅳ reached the peak at day10and then declined, but the that oftreatment group Ⅰreached the peak at day20and declined later. There was no obviousdifference (P>0.05) on serum Se levels between treatment Ⅲ and treatment Ⅳ,but thedifferences between treatment groups and control group were obvious after day10or day20(P<0.05). even though the serum Se of treatment groupsⅠdropped to the lowest level(86.79±3.57μg/L), it was still significantly more than that of the control group(19.87±4.18μg/L)(P<0.05) at day50.(3) The serum BUN concentrations of treatment group Ⅰ,Ⅱ,ⅢandⅣwere8.79±1.15,8.27±3.12,8.89±2.46and7.79±2.03mmol/L, respectively on50thday. All of which weresignificantly lower than that of the control group (10.56±2.31mmol/L)(P<0.05), butindividual differences among treatment groups were not significant (P>0.05). In the rest of thestage, the BUN levels of treatment groups were lower than that of the control group, with nodifference between the treatment groups (P>0.05).The Cr levels of treatment goats were lowerthan the control group (P>0.05).(4) There were no any significant differences on serum CHO and TG levels (P>0.05)among all the groups at day0-3. The serum CHO levels of all treatment goups weresignificantly lower than the control groups (P<0.05), and there was no significant differencebetween treatment groups (P>0.05) at10-50days. The serum TG levels of group Ⅰ, Ⅱ, Ⅲ,Ⅳ were0.176±0.048,0.160±0.036,0.190±0.025and0.208±0.024mmol/L, respectively, allof them were significantly lower than the control group (0.276±0.036mmol/L)(P<0.05). Theserum TG levels of groupⅠ, Ⅱ significantly lower than group Ⅲ, Ⅳ(P<0.05), but therewas no significant difference(P>0.05) between groupⅠand Ⅱ, Ⅲ and Ⅳat day10. Atday20, the serum TG levels of all treatment goups were significantly lower than the controlgroups (P<0.05), and the serum TG content of group Ⅱ was significantly lower than group Ⅰ, Ⅲ and Ⅳ(P<0.05), and there were no significantly among the group Ⅰ, Ⅲ and Ⅳ(P>0.05). The serum TG levels of all treatment goups were significantly lower than thecontrol groups (P<0.05), and there was no significant difference between treatment groups(P>0.05) at30-50days. During the test, The difference among blood sugar levels of all groupshad not been found(P>0.05).(5) Serum T-SOD levels of treatment groups increased, but there was no significantdifference compared with the control group (P>0.05) at day0-3,but all of them were above218.66±22.84U/mL, that were significantly higher than the control group(P<0.05) atday10-50.(6) The muscle selenium content of treatment groups were significantly higher than thatof the control group (P<0.05). The order of selenium content was groupⅣ (0.183±0.036mg/Kg)> group Ⅲ (0.126±0.022mg/Kg)>group Ⅱ (0.089±0.019mg/Kg)> group Ⅰ(0.058±0.023mg/Kg)> control (0.030±0.017mg/Kg). More specifically, the muscle seleniumcontent of group Ⅲ and groupⅣ were significantly higher than that of group Ⅰand Ⅱ(P<0.05). The drop loss of group Ⅱ (3.78±0.19%) was significantly lower than the othergroups (P<0.05), and there was no significant difference in drop loss among the other groups(P>0.05). The effect of sodium selenite on crude protein, crude fat, ash and moisture contentwere not significant.In sum, the serum BUN, TG and CHO levels and muscle drip loss of the castrated Boergoat crossbred kids were reduced by injecting sodium selenite. while the serum T-SOD, serumSe levels and muscle Se content were increased. |
PDF Full Text Request