The Study Of The Effects Of The Self-made TCM Ointment On The Soft Tissue Injured Of Mouses

TCM ointment for external use, developed in accordance with senior professors’ proved recipe, is a pure traditional Chinese medicine preparation applied to treat the injured soft tissue of horses’ hooves. This research established a theoretical foundation for clinic application and promotion by observing its anti-inflammatory and analgesic effects as well as morphologic changes, and exploring the anti-inflammatory mechanism.Under experimental conditions, a rat model of toe swelling, an ear-swelling model, an acetic acid induced pain model and a model of pain induced by hot plate were respectively established to observe anti-inflammatory and analgesic effects of self-made TCM ointment for external use. Meanwhile, a model of increased capillary vessel permeability and a hemorheological model were built to examine the blood-activating and stasis-eliminating effects. Furthermore, the model of acute soft tissue injury was developed to verify the changes in movement of all model groups’ animals and morphologic changes after using the ointment. Then, ELISA test kit was used to test the content of inflammatory factors including TNF-α, NO, COX-2, and HIS in each model group’s injured soft tissue so as to explore the anti-inflammatory mechanism of the self-made TCM ointment for external use.Experimental results were:(1)both the high-dose and medium-dose TCM ointment could significantly inhibit the mouse ear-swelling incurred by dimethylbenzene and toe-swelling resulted from egg-white injected (P<0.01). Besides, high-dose, medium-dose and low-dose groups could remarkably raise the mice’pain threshold value when the hot plate method and acetic acid writhing method were applied, which indicated that this ointment has good analgesic effects (p<0.01).(2)high-dose, medium-dose and low-dose groups could effectively reduce the capillary vessel permeability caused by the reduction of acetic acid and obviously decrease the hemorheological indexes such as whole blood viscosity and plasma viscosity (P<0.01), which suggested that this ointment has blood-activating and stasis-eliminating effects.(3)With regard to histomorphology, comparisons were made between high-dose, medium-dose groups and model groups after using the ointment. It was found that the stasis was greatly eliminated and muscle fiber was repaired comparably more rapidly. Despite the pathological changes of low-dose groups were similar to those of model groups, the stasis and muscle fiber swelling was relatively slighter. Moreover, the histopathological scores of high-dose, medium-dose, low-dose groups and model groups obviously differed from each other in each time point.(4)Each drug guoup could lower the contents of NO, COX-2, TNF-α, and HIS (P<0.01) than model groups in the mices’ soft tissue injured. Moreover, the reduction of such contents was somewhat dose-dependent, but it was of no statistical significance to com-pare with positive drug groups (P>0.05).Conclusions:The self-made TCM ointment has good effects for treating the soft tissue injured and has therapeutic anti-inflammatory, stasis-eliminating, blood-activating as well as analgesic effects.