| This study was conducted to investigate the effects of different dosage of compound traditional Chinese medicine" Zhukangsan " on humoral immunity and cytokine in piglets, and also intended to explore the mechanism and the best Feeding dose of " Zhukangsan " for developing a organic high performance traditional Chinese immunopotentiator.40D*L*Y crossbred piglets were randomly assigned to five groups, each group was8piglets and the study lasted45days. All piglets were vaccinated two dose of classical swine fever vaccine at21days old. The positive control group were injected0.02ml/kg BW transfer factor. During the27to31days old, the negative control group and the positive control group were fed basic ration, the Ⅰ,Ⅱ,Ⅲgroup were respectively fed basic ration and " Zhukangsan "0.5%,1.0%,2.0%. The blood samples were respectively collected at35,45,55and65days old for analyzing B lymphocytes, antibody level of swine fever, Immunological protein(IgG, IgA and IgM), complement body(C3and C4), IL-2, IL-6, IFN-γ and TNF-α.The resluts indicated that (1) the traditional Chinese medicine " Zhukangsan’ and the TF both improved the proliferation of B lymphocytes. Transfer factor group and the Ⅱ group all showed the significant effection, but the latter showed the most significant effection.(2)" Zhukangsan " could enhance the production of swine fever antibody and prolonged the antibody time, the Ⅱ group showed the best significant effection, the transfer factor group and the Ⅲ group take second place.(3) Zhukangsan " can enhance immunoglobulin IgG, IgA and IgM of piglets in serum significantly, the Ⅱ group showed an optimal effection on improving immunoglobulin levels during the whole experiment;The content of IgM were enhanced ignificantly of group Ⅰ and Ⅲ at the age of45days old(P<0.05); The content of IgG were enhanced ignificantly of group Mat the age of35and45days old(P<0.05);Transfer factor group showed the significant increase of the content of IgG at the age of35days old(P<0.05).(4) The Ⅱ group showed the significant increase of the content of serum C3, C4; The Ⅰ、Ⅲ group showed the significant increase of the content of the complement C4at the age of55days old(P<0.05); In a certain extent, application transfer factors can improve the complement C4content, but there is not significant difference to C3content.(5) The Ⅱ group the significant increase of the levels of IL-2、IL-6、IFN-γ and TNF-α in the blood serum; Compared with the negative control group, the levels of IL-2in group Ⅰ enhanced ignificantly at the age of35days and45days old(P<0.05) and in group Ⅲ enhanced ignificantly at the age of55days old(P <0.05); The Ⅰ and Ⅲ group showed the significant increase of the levels of IFN-γ,but the levels of IL-6and TNF-α in group Ⅰ、Ⅲ is not significant difference compared with the negative control group. Application transfer factors can significantly enhance the levels of IL-2、IFN-γ,but the effection was inferior compared with group ⅡIn conelusion,"Zhukangsan" can improve piglets humoral immune function, enhance the serum cell factor levels.1.0%is the best Feeding dose of "Zhukangsan" which is better than transfer factor. |
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