| The angelica dahurica of “Yu Baizhi” is a kind of Chinese herbal medicine, whichis used frequently in medicine and life. The main ways of processing in productionplace is drying in the sun and stoving, so its added value is low. However, Yu Baizhi isperishable easily in storing for a large count. Sometimes sulfitation was use to improvethe quality of angelica dahurica and extend its storage period. The paper made a studythat angelica dahurica was cut into slices to dry and the total coumarin content in theseslices was determine after drying, then the effects were observed. This could provide abasis for industrialization and packet in production place. That the extraction andpurification methods of coumarins in angelica dahurica were studied could improvethe yield and purity of medicinal ingredients, and provide a reliable basis for angelicadahurica development and utilization.In this paper, Yu Baizhi angelica dahurica was the main study material. Firstly,the slices of Yu Baizhi root were dried, and then the results were observed whether thedry methods under different temperatures had some effects to coumarin content. Andthe total acidity, total phenols, total sugar and protein in Yu Baizhi were determined indifferent ways. Finally the total coumarin in Yu Baizhi were extracted, separated andpurified. The main research contents are as follows:1. Comparing the main component coumarin content in angelica dahurica slicesafter drying, found that the suitable temperature range was40-70℃, and the contentof total coumarin was higher in this temperature range. With the drying temperaturebecoming higher, the content decreased obviously.2. The content of total sugar, protein, total acidity and total phenol in Yu Baizhiroot were measured, and the content was respectively34.413%,9.92%,1.72%and0.679%. These components accounted for about46.7%in Yu Baizhi, and that choosingan appropriate extraction and separated methods to remove these impurities could provide the foundation basis for the separation and purification of the total coumarin.3. The heating reflux and soxhlet extraction method were used to extract the totalcoumarin in Yu Baizhi. The orthogonal test of optimizing the extraction process wasrespectively as follows: heating reflux extraction process was that the extractiontemperature was70℃, the solid-liquid ratio was1:20, the times of extraction was3times, the time was2h, and the average extraction rate was0.638%; soxhlet extractionprocess was that95%ethanol, the temperature of85℃, extraction time of5h werechosen, and the average extraction rate was0.581%.4. D-101macro porous resin was chosen as a better resin for purification of thetotal coumarin. The orthogonal test of its purification process was that adsorption flowrate of1mL.min-1, eluent ethanol concentration of80%, eluent volume of6BV volume,elution rate of2mL.min-1were chosen, and the average content of coumarin was78.73%, the purity was72.71%on average. The alkali-soluble acid precipitation wasused to separate and purify the total coumarin from the ether extract, and the averagecontent of coumarin was57.19%, the average purity was88.24%. Compared with theresin adsorption, the method of alkali-soluble and acid precipitation operation wasmore complicated, and the average content of coumarin was relatively low.The UV spectrophotometric method was built to determine the total coumarincontent of angelica dahuarica. Then the drying parameters were obtained，and theeffects on the total coumarin content were analyzed. The technologies of the extractionprocess and purification process were studied, and could provide the foundation basisfor further research or processing in Yu Baizhi. |
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