The Impact Of Early Young Stage Swimming On The Hypothalamic Npymrna And POMCmRNA Of Adult Rats With High-fat Diet

Obesity is a kind of common disease in modern society.Obesity, with a complicated pathogenic mechanism, contribute to varying hazards of each organization and shortened survival time to a certain extent. Studies have shown that obesity is a nutritional aplastic disease caused by metabolic and appetite disorder. It not only related to the modern living environment and dietary structure but also connected with many factor of gene.At present, hypothalamus is identified as the control center of ingestion and energy balance, and it is also the centrum of weight regulation, in which food intake and weight gain are dominated by nucleus neurons NPY and POMC of ARC. Earlier studies demonstrated that exercises during young stage could make the body fat accumulation reduce and there was no bounce back later, which may directly relate to ennegy homeostasis regulated by the cooperation of hypothalamus neuropeptide systems.But infancy obesity mostly tended to be preservered into adult, so ahead of the focus of the work, developing intervention strategies for obesity became very important. During the body growth period, it is recommended to take exercises to resist and prevent series of body damage because of obesity induced by high fat diet.This paper observing the influence of swimming during the body growth period on body fat and expression levels of NPY mRNA and POMC mRNA of hypothalamus caused by fed with high fat diet, which was aimed at discussing whether the early exercises had trace effect and protective action on adult body with obesity induced by high fat diet.Methods:Thirty3-week-old rats were randomly assigned to quiet control group (C) and exercise group (E) with regular diet feeding.Group E rats trained with no load but time increase swimming for9weeks,45min/d,6d/week. At12-week-old, group C rats were randomly assigned to the regular diet feeding control group (CC) and the high fat-diet control group (CH). Group E was fed by high-fat diet (E9H) and drawn material at24-weeks-old after stopped exercising. Measured the rats weight, food intake, fat pad weight, corpus adiposum ratio and the expression levels of the hypothalamic NPY mRNA and POMC mRNA.Results:①The9weeks swimming on early young stage intervention, compared with group E and group C, weight (413.50±39.40VS423.66±35.02)and the fat pad weightand corpus adiposum ratio of group E rats’ visceral (7.06±1.25VS11.39±1.11;0.96±0.24VS2.54±0.41) are observably lower than group C. However, did not cause the significant difference (P>0.05) on food intake (1419.30±85.73VS1418.20±72.73). The hypothalamic NPY mRNA and POMC mRNA of group E rat is higher than group C and the difference is remarkable(1.13±0.44VS1;1.78±0.91VS1;P<0.01).②At the end of12weeks fatty meal among adult stage,compared with group CH and group CC, weight (641.33±65.03VS581.00±67.29)and the fat pad weightand corpus adiposum ratio of group E rats’ visceral (29.62±5.89VS21.16±7.10;4.60±0.74VS3.51±1.01) are observably higher than group CC. However, did not cause the significant difference (P>0.05) on food intake (2051.53±204.78VS2291.03±210.20),but the calories intake higher than group CC(38979.17±3890.93VS29783.49±2732.72; P<0.05). The hypothalamic NPY mRNA and POMC mRNA of group E rat is higher than group CC and the difference is remarkable(3.26±1.81VS1;2.06±0.51VS1;P<0.05).③At the end of12weeks fatty meal among adult stage, compared with group E9H and group CH,weight (555.18±66.87VS641.33±65.03) and the fat pad weightand corpus adiposum ratio of group E9H rats’ visceral (7.64±2.76VS29.62±5.89;1.61±0.59VS4.60±0.74; P<0.01) are observably lower than group CH. Did not cause the significant difference (P>0.05) on food intake(2079.64±298.69VS2051.53±204.78)and the calories intake(39513.26±5675.13VS38979.17±3890.93) and the hypothalamic NPY mRNA(2.29±0.57VS3.26±1.81) and POMC mRNA(1.47±0.49VS2.06±0.51) Conclusions:①The9weeks swimming on early young stage can effectively reduce the body fat of rats and improve the corpus adiposum ratio.②The9weeks swimming during the infant period upregulated NPY mRNA and POMC mRNA in the rat’s hypothalamus. Exercises during infancy increase central suppress appetite neuropeptide mRNA.③The12weeks high-fat diet on adult stage increased the hypothalamic NPY and POMC. May be the weight gain which is caused by the high-fat diet can bring out the protective reaction.④Swimming at early young stage has a certain degree influence on central apetite neuropeptide of the one with high-fat diet,but the protective function of exercise has yet to be further researched.