Culture In Vitro Of The Medicinal Plant Sophora Flavescens

In this experiment, the tender leaves, stem segments, immature embryo and hypocotyls of Sophora flavescens as explants, a set of propagation system in vitro about the induction of callus tissue, differentiation, proliferation and rooting was established. It provided a theoretical basis for the industrial production of Sophora flavescens. The main results are summarized as the followings:(1)The best methods of disinfection for different explants:the stem segments were treated with75%alcohol for30to40S, sterile water for several times, and then0.1%HgCl2for6min. The leaves were treated with75%alcohol for30to40S, sterile water for several times, and then used0.1%HgCl2for4min. The seeds were treated with75%alcohol for30to40S, sterile water for several times, and then0.1%HgCl2for8min.(2)The optimal culture medium for the callus induction of Sophora flavescens stem segment explants was MS+2,4-D1mg/L+6-BA2mg/L, and the highest induction rate reached98.9%. It was found that the callus tissue which was produced by the stem segments sticked upright into MS+NAA0.5mg/L+6-BA0.5mg/L had a strong differentiation ability. The optimal culture medium for the leave callus induction was MS+6-BA1mg/L+2,4-D2mg/L, and the highest induction rate was91.1%. The most suitable medium for the immature embryo callus induction was MS+6-BA1mg/L+2,4-D0.5mg/L. The most suitable medium for the hypocotyl callus induction was MS+6-BA0.1mg/L+2,4-D0.5mg/L.(3)In this experiment, it was found that the most suitable temperature of the stem segments callus induction was24℃～26℃, the callus induction rate had reached96.3%, and the callus browning was not easy to produce. It was showed that the effect of the light on the leaf callus:in the dark, the callus surface had the loose white granular material and internal solid; then in the light, the callus was turned to green, and the texture was more loose.(4)The stem segments were sticked upright into MS+NAA0.5mg/L+6-BA0.5mg/L and induced to produce the callus tissue which had a strong ability of the adventitious bud differentiation. The optimal culture medium for the adventitious bud differentiation was MS+6-BA2mg/L+NAA0.5mg/L.(5) In multiplication stage, MS medium supplied with IBA0.5mg/L+6-BA2mg/L was the best culture medium, on which the bud differentiation rate and multiplication rate were higher, and bud had strong growth. After subculture a large number of seedlings were obtained in a short time.(6)The rooting rate reached81.3%on the medium1/2MS supplied with IBA1.5mg/L. Moreover, the suitable proportion of transplanting medium of Sophora flavescens with peat soil, perlite and vermiculite was3:1:6, and the transplanting survival rate was the highest of90%.