Studies On Bacteria Culture And Feature Of The Histopathological And Immunohistochemical Staining In The Wall Of The Uterus Of43Canine Pyometra Cases

Pyometra is a bacterial infection of the uterus that is common in dogs and is potentially life-threatening if delayed in diagnosis and/or treatment.The study was performed at the animal hospital of YangZhou university from January2012to March2014. A case study of20dogs with closed cervical pyometra and23dogs with open cervical pyometra and17healthy control female dogs was used to investigate the relationships between age, breed, season, time after the heat and reproductive history; Routine blood and blood biochemical changes; Bacteria cultivation of pyometra cases; Histological features with HE staining and TLR2、TLR4、ER and PR were examined immunohistochemically in uteri of control bitches,closed cervix pyometra and open cervix pyometra.Cases were reported in local city with a mean onset age of9.2±2.6years old, The incidence of pyometra above6years age accounted for90%. The Pekingese and Pomeranian had an increase risk of developing pyometra. The Pekingese makes39.5%and Pomeranian makes16.3%of all cases. The onset time of disease was in average1.5±0.4month after oestrus.study also reavealed that most pyometra developed month were from April to July (67.5%) and September to November (22.6%). Previous pregnancy was statistically associated with pyometra, nulliparous bitches accounted for81.4%of all pyometra cases.Blood samples obtained from cephalic vein before ovariohysterectomy for blood routine and blood biochemical detection. The WBC and Gran of closed cervical and open cervical pyometra were higher than the control group, and extremely significant difference (P<0.01); The WBC and Gran of closed cervical pyometra were higher than the open cervical pyometra, and significant difference (P<0.05). The RBC and HGB of closed and open cervical pyometra group were lower than the control group, and significant difference. BUN and CREA in serum were significantly higer in dogs with closed cervical pyometra as compared with control dogs and open cervical pyometra (P<0.05), Kidney damage might be related to polydipsia and polyuria of closed cervical pyometra. GLO in serum were extremely significantly higer in dogs with closed and open cervical pyometra as compared with control dogs (P<0.01).Sample for microbiological culture were collected from the pus after ovariohysterectomy, sample were cultured for aerobic and anaerobic bacteria in ordinary agar medium, MaiKangKai agar medium and blood agar. Microbiological tests revealed Escherichia coli and Pseudomonas aeruginosa in43bitches respectively makes52.6%and18.4%of all cases and also we isolated Klebsiella pneumonia, Serratia marcescens, Enterococcus faecalis, Staphylococcus epidermidis, Staphylococcus aureus.Histological features of pyometra that present in most case are endometrial cysts. these exuberant endometrial secretions likely contribute to establishment of bacterial infections and associated inflammation that can develop into pyometra, there is a neutrophilic cellular inflammatory initially involving the superficial endometrium and Glandular epithelium.The immunohistocheical staining of TLR2and ER density of all pyometra uterine cell types were lower than the TLR2and ER density of normal uteri in the dioestrus. The TLR4and PR density of all pyometra group were significantly higher than the TLR4and PR density of the normal uteri. The TLR4density was significantly higher than the TLR2density in the pyometra group. The PR density was significantly higher than the ER density in the pyometra group.In conclusion, canine pyometra was charactered with elevated WBC and decreased RBC, it could cause renal damage and e.coli and pseudomonas aeruginosa is the most commonly isolated bacteria, endometrial cystic hyperplasia and TLR4and PR significantly expression were in the endometrium.