Ganoderma Lucidum Spore Extract Different Effective Parts And In Vivo Antitumor Research

Ganoderma lucidum spores is mature ganoderma lucidum growth from pileus eject extremely tiny spores,reproductive cells for ganoderma lucidum,all the genetic active substances with ganoderma lucidum.Modern pharmacological research show that the ganoderma lucidum spores has anti-tumor,enhance immunity,anti-virus,anti-inflammatory,fall blood sugar,fall hematic fat,resisting lack of oxygen capacity,scavenging free radicals,and so on.Ganoderma lucidum and ganoderma lucidum spores chemical composition is complicated,has extensive biological activities,including polysaccharides and triterpenes two kinds of active substance is considered to be main efficacy components.Ganoderma lucidum spore oil is ganoderma lucidum spores by the broken wall,supercritical carbon dioxide extraction from fat soluble active substances,anticancer,modern research has shown that protect liver to protect liver,enhance immunity,etc.Previous research that ganoderma lucidum spore oil with immunity and anti-tumor effect is due to its rich in ganoderma triterpene compound.With development of further research of ganoderma lucidum spores oil components in recent years,more and more research suggests that ganoderma lucidum spore mainly exist in triglycerides compounds,and a small amount of free fatty acids,steroids,esters,fat-soluble vitamins,only contains trace amounts of triterpene composition.Through different process to extract the different effective parts of ganoderma lucidum spores,including mainly the ganoderma lucidum polysaccharide of ganoderma lucidum spores aqueous extract,ganoderma lucidum triterpenes and sterol mainly alcohol extract of ganoderma lucidum spores,supercritical carbon dioxide extraction from lipid effective parts of ganoderma lucidum spore oil.With ultraviolet-visible spectrophotometry and HPLC method for determining the efficacy of different effective parts composition;And passed the test of the liver cancer H22 cell lines underarm inoculated nude mice,the antitumor function of the ganodermalucidum spore different effective parts.For ganoderma lucidum spores different effective parts to provide technical support to further the development of drugs has clinical value.The research content is as follows:1.The ganoderma lucidum spore different effective parts extraction technology was studied?1?water extract single factor experiment?2?the alcohol extraction of single factor experiment?3?the fat-soluble effective parts?ganoderma lucidum spores oil?,supercritical CO₂ extraction of single factor test and orthogonal test2.The ganoderma lucidum spores different effective parts composition determination?1?the ultraviolet-visible spectrophotometry terpene and sterol content?2?the ultraviolet-visible spectrophotometry polysaccharide content?3?the HPLC method for determination of triglyceride component content and methodology test3.The different parts of ganoderma lucidum spores in vivo antitumor researchBy liver cancer H22 cell lines underarm inoculated nude mice experiment of ganoderma lucidum spores water extract,ganoderma lucidum spore alcohol extract,ganoderma lucidum spore lipid parts?such as ganoderma lucidum spores oil?the antitumor function of the different effective partsThe results of the study are as follows:1.Adopt heating refluxing extraction,aqueous extract optimum upon maturity of lingzhi only for the material liquid than 1:30,extracting time for 2h,extraction times,temperature 100?.2.By ultrasonic extraction,alcohol extract optimum upon maturity of lingzhi only for the material liquid than 1:30,extracting time for 2h,extraction number 1 time,extraction temperature 60?.3.The ganoderma lucidum spores oil supercritical CO₂ extraction optimumprocess for extraction pressure 25 mpa,extraction temperature 40?;Separation tank pressure I to 10 mpa,temperature 45?.Three times of extraction process validation results for 48 g oil,oil recovery rate was 24%.Extraction process stability is feasible and has good repeatability.4.The ganoderma lucidum spores alcohol extract mainly terpene and steroid composition,content of 26.8%,ganoderma lucidum polysaccharide content is 3.24%.Ganoderma lucidum spores aqueous extract is given priority to with polysaccharide,content of 16.96%,terpene and sterol content 1.05%.Ganoderma lucidum spore oil is given priority to with triglycerides,content of 74.25%,terpene and steroid component content of 35.51%5.Ganoderma lucidum spore oil?oral?group,ganoderma lucidum spore oil?injection?can significantly inhibit nude mice H22 liver cancer growth effect?compared with control group,P<0.05 or P<0.01?,the inhibition rate of 30.78%,48.14% respectively;Ganoderma lucidum spores water extract,ganoderma lucidum spore alcohol extract also has certain inhibition,but compared with the control group no significant statistical difference?P>0.05?,inhibition rate of 17.76%,26.29%respectively;With ganoderma lucidum spores oil oral and injected tumor suppressor effect is best.