Establishment Of Tissue Culture System Of Padus Virginiana Study On The Change Of Pigment Content In Leaves And Plantlets

Padus virginiana is an important color-leaf tree species in Northeast my country,and it is also a high-quality tree species for landscaping.It has excellent characteristics and high ornamental properties.In order to meet the demand for greening seedlings in garden production,this study used the semi-lignified stems and leaves of Padus virginiana as explants,and discussed the effect of disinfection time,basic medium type,hormone type and concentration ratio on different parts of Padus virginiana for the influence of explants,the best culture conditions for Padus virginiana were screened out,and a complete Padus virginiana rapid propagation system was established,which provided a theoretical basis for Padus virginiana tissue culture technology;at the same time,it provided a thorough understanding the characteristics of Padus virginiana leaf color change,determine the relevant pigment content of tissue cultured seedlings,analyze the best combination of four environmental factors for the change of pigment content and accelerate the change of leaf color,make the viewing period longer,and increase the characteristics and speed up the discoloration period provides theoretical support.The research results are as follows:1.With the stems of the Padus virginiana as explants,sterilize with 70%alcohol for 30 s and 0.1%Hg Cl₂ for 10 min?12 min and 14 min.The optimal disinfection time is selected as12 min,and then the aseptic culture system of Padus virginiana was established.Use MS and WPM medium as the basic medium,add the same hormone concentration respectively,and select the best medium as MS medium;for primary culture,use MS as the basic medium and add different concentrations of 6-BA?NAA?IBA found that the best medium is MS+0.5 mg/L6-BA+0.05 mg/LNAA;the proliferation medium is MS+0.8 mg/L 6-BA+0.08 mg/L NAA for proliferation The effect is the best,the proliferation coefficient is 5.22;the test-tube seedlings are inoculated into the rooting medium to induce rooting,and the best rooting medium is selected as 1/2 MS+0.5 mg/L NAA,and the rooting rate reaches 75%;After seedlings,it is transplanted into a matrix of perlite:vermiculite:peat soil=1:1:1,and the survival rate reaches more than 80%.2.Use Padus virginiana tissue culture seedling leaves as explants for callus induction,MS medium as basic medium,and add different plant growth regulators 6-BA?NAA?2,4-D?TDZ,and screen out The best medium combination is MS+1.0 mg/L 6-BA+0.5 mg/L NAA+1.0 mg/L 2,4-D,and the recovery rate is 70.8%.3.Obtain regeneration tissue culture seedlings through rapid propagation in vitro,take the leaves of the tissue culture seedlings at the growth stage,measure the leaf pigment content under different environmental conditions,and determine the effect of the WPM?MS medium type on the leaf pigment content and growth status during the tissue culture process Out:WPM is more suitable for the growth environment of leaves than MS,and has better growth.At the same time,5 hormone combinations are set to determine the pigment content,and the measurement results are 0.5 mg/L 6-BA and 0.05 mg/L NAA.The rapid discoloration and growth of the leaves of tissue cultured seedlings produced the best results;when the sucrose content was 20 g/L?25 g/L?30 g/L,20 g/L was selected as the most suitable Padus virginiana tissue culture seedlings.The discolored sucrose concentration produces the best results;when the pigment content is measured under 40%,60%,and 100%light conditions,it is found that the leaves have different content changes and it is concluded that the 100%light leaves are more effective than the other two light conditions.The leaves of Padus virginiana tissue culture seedlings are more suitable.