| Objective: To observe the effects of acupuncture method of "Guben peiyuan,Jianpi hewei" on m TOR pathway in simple obese rats induced by high fat diet.Methods: In this study,60 male SD rats were randomly divided into blank group with 10 rats.The rest of the rats were induced to become simple obese rats by high-fat diet.According to the principle of randomization,they were divided into three groups: model group,rapamycin group,acupuncture group,with 10 rats in each group.Rats in blank group were fed with common diet,while rats in the other three groups were fed with high-fat diet.The binding adaptability of 4 groups of rats was trained for 2 weeks.After the binding training,the rapamycin group was given rapamycin solution by gavage;the acupuncture group was given acupuncture with the method of "Guben peiyuan,Jianpi hewei".During the treatment,The survival status of rats was observed regularly and the rat body weight was inspected regularly.After 30 days of intervention,sample were taken after fasting for more than 12 hours,and the blood glucose,TG,TC contents in each group were detected.The expression levels of m TOR,Raptor-m TORC1,p S6K1 and p IRS-1 in liver tissues of rats in each group were detected by Western Blot.Results:(1)Rat survival status: no abnormalities occurred in the rats of each group during the experimental intervention.The blank group was in good mental state,with sensitive activities,and could quickly respond to externalstimuli,eyes are spirited,and there was no abnormal smell or color change in both defecates.The fur was white and shiny.Compared with the normal rats,the rats with successful modeling were not in a good mental state,with more sleeping and less moving,a slow response to external stimuli,dull eyes,and sticky stools,hair loss,poor color and obvious body obesity.After 30 days of intervention,the rats in each group showed good improvement compared with their previous physiological states(external stimulation,physical appearance,food intake and drinking water,hair color,urine and feces,and activity).Moreover,the acupuncture group was better than the rapamycin group in terms of external stimulation,body shape,defecation and hair color improvement.(2)Before intervention: the body weight of rats in the model group was significantly higher than that in the blank group,with a significant difference(P<0.05).There was no significant difference in body weight among model group,rapamycin group and acupuncture group(P>0.05).After intervention:the weight of the rapamycin group and the acupuncture group decreased significantly compared with the model group(P<0.05),and the weight of the acupuncture group decreased more significantly than that of the rapamycin group(P<0.05).(3)Blood glucose,TG and TC in the model group were significantly higher than those in the blank group,with significant differences(P<0.05).After intervention: blood glucose,TG and TC in the rapamycin group and the acupuncture group were all reduced compared with the model group,with significant differences(P<0.05).There was a significant difference between the acupuncture group and the rapamycin group in the reduction of blood glucose and TC(P<0.05),while there was no significant difference between the acupuncture group and the rapamycin group in the reduction of TG(P>0.05).(4)The expression levels of m TOR,Raptor-m TORC1 and p S6K1 in the model group were significantly higher than those in the blank group,the difference was statistically significant(P<0.05),After intervention: the expression levels of m TOR,Raptor-m TORC1 and p S6K1 in the liver of rats in the rapamycin group and the acupuncture group were significantly lower than the model group(P<0.05).The acupuncture group was lower than the rapamycin group,and the difference was not statistically significant(P>0.05).(5)The expression levels of p IRS-1 in the model group were significantly lower than those in the blank group,the difference was statistically significant(P<0.05),After intervention: the expression levels of p IRS-1 in the liver of rats in the rapamycin group and the acupuncture group were significantly higher than the model group(P<0.05).Moreover,the acupuncture group had a more significant decrease than the rapamycin group(P<0.05).The acupuncture group was lower than the rapamycin group,and the difference was not statistically significant(P>0.05).Conclusion:(1)In this study,the acupuncture method of "Guben Peiyuan,Jianpi Hewei" was adopted to significantly improve the body weight,blood glucose and blood lipid levels of simple obese rats.(2)Rapamycin can inhibit the m TOR pathway in obese rats,thus improving insulin resistance and treating obesity.(3)Acupuncture may have a similar mechanism to rapamycin,which can improve insulin resistance by inhibiting the m TOR pathway and phosphorylation of downstream S6K1,and treating obesity.(4)There was no significant difference between acupuncture and rapamycin in inhibiting the m TOR pathway and improving the levels of m TOR,Raptor-m TORC1,p S6K1 and p IRS-1.However,combined with thecomprehensive comparison of the results of weight,blood glucose and blood lipid in each group,the curative effect of acupuncture on simple obesity was better than rapamycin. |
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