| The secondary class national preserved and endangered plant Parakmeria lotungensis (Chun et C.Tsoong)Law, The first class national preserval plant Sinomanglietia glauca Z.X.Yu et Q.Y.Zheng endemic in Jiangxi province, and the up-to-date and rare color-leaf plant Photinia fraseri 'Red Robin' were respectively studied to establish tissue culture and rapid propagation system based on plant cell totipotency and its regulation and control theory. Meanwhile, the regeneration mechanism of tissue culture of P. fraseri was discussed by means of anatomy observation and enzyme activity measuration related with rooting. The bio-antisepsis was used in controlling the contamination in plant tissue culture. In addition, by combining with techniques of horticulture, the systematized techniques of rapid micropropagation of woody plant via tissue culture were specially investigated. The results were listed as below:The regeneration system of tissue culture of P. lotungensis was first established by utilizing its twigs from the mature tree as explants. In the first ten days of October, the eugonic twigs were excised from mature tree, and pretreated them with low temperature (4℃) for 5-7d in order to decrease brownness and contamination, and increase survival rate of induced propagation. MS was more effective than the other as basic medium in the whole experiment. The best medium of proliferation was MS+6-BA0.2mg/l + IBA0.05mg/l+30g/l Sucrose +7g/l Gelatin, pH5.5-6.5, illumination intensity 1000 Lx, subculture interval was 4 weeks. After subculture for 8 times, the proliferation was fast and stable, and multiplication rate was 3.5. The buds 1.5-2cm long were planted into root-induced medium (1/2MS+NAA3.0 mg/L +BA0.1 mg/L), after 15d under the darkness, and by transferred into rooting medium (1/2MS) without any hormone, rooting was started at about 12days, and the rooting rate was 15-25%.The matter E was first used in tissue culture root induction. The additive matter E makes a full impact on adventitious root formation of P. lotungensis. The rooting rate has reached 77% , and much more 50% have been increased than comparison,The regeneration system of tissue culture of S. glauca was established by utilizing its twigs and dormancy bud as explants. the eugonic twigs excised in the first ten days of October, and dormancy excised in March were fit for induction of bud. M * was more effective than the other as basic medium. The best proliferation medium was MS+6-BA0.5mg/L+IBA0.05mg/L+30g/lSucrose+7g/lGelatin, pHS.8, illumination intensity lOOOLx, subculture interval was 10 days, The buds 1.5-2cm long were planted into root-induced medium 1/2MS+NAA3.0 mg/L +BA0.1 mg/L without Gelatin but with perlite or vermiculite , afterlO-12d under the darkness, andby transferred into root-formation medium (l/2MS)without any hormone, rooting was started at about 12-15days.This study established an economic and hight effective technology system on rapid propagation of P. fraseri 'Red Robin ' .By comparision of the mainly factors on induction culture proliferation culture rooting culture and transplant culture , the optimal inducing medium was MS+KT1.0mg/l+NAA0.2mg/l; the optimal multiplication medium was MS+6-BA2.0 mg/l+KT0.5mg/l+IBA0.2mg/l+30g/lsugar.+7g/lGelatin, feasible illumination intensity was 3-5d dark culture combined with 1000-15001x light culture 12h/days, the highest multiplication rate was 9.3. On average, there were 32 shoots white can be used for rooting in one bottle.M medium and NAA was effective on rooting of P. fraseri, and the rooting rate was 66% after 20days by incubation on medium M *+ NAA0.3mg/L.The additive matter E makes a full impact on adventitious root formation of P. fraseri. The rooting rate has reached 98.5% and much more 30% have been increased than comparison, and at the same time ,root grow fast and order; It farther demonstrates in theory that appending substance E or not has an straight effect on the rooting rate and time of root emergence through the examinations of anatomy observation and enzyme activity measuratio...
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