Radish Seed Water-soluble Alkaloids Of Apoe <sup> - / - </ Sup> Mouse Vascular Endothelial Protection Mechanisms Experiment Study

Cardiovascular or Brain disease due to atherosclerosis is the major cause of mortality and morbidity. Atherosclerosis is characterized by the presence of atherosclerosis, leading to artery intima lumen stenosis. Risk factors of atherosclerosis include elevated low-density lipoprotein-cholesterol levels, low high-density lipoprotein-cholesterol levels, elevated TG levels, obesity, hypertension, smoking, diabetes, and genetic factors,et al. The incidence of the disorder related to lipid metabolism in vivo and vascular endothelial dysfunction in such areas, Because of its molecular mechanism of disease are more so on a single target in the treatment than the treatment of multi-target synergy. Therefore, the search for effective therapeutic approach of Chinese medicine, which plays a great significance to regulate advantage.Tutor guo zhong gai Professor thought the key of this disease is ben xv biao shi, The most important pathogenic factors is tan zhuo yong zhi, Radish Seed clinical application of anti-atherosclerosis reach good effect.and it can play jiang qi hua tan,tong fu xie zhuo role. To further clarify the anti-atherosclerosis effect and related mechanisms of Radish Seed, prove objective, scientific evaluation, We extract the active ingredients of the Radish Seed,which is soluble semen raphani alkaloids,And used ApoE knockout (ApoE-/-) mice for the study, carried out the following studies:1. Soluble semen raphani alkaloids effects on the ApoE-/- mice with plasma lipid levels;2. Soluble semen raphani alkaloids effects on the serum nitric oxide (NO), nitric oxide synthase (NOS) ;3. Soluble semen raphani alkaloids effects on the ApoE-/- mouse serum superoxide dismutase (SOD), malondialdehyde (MDA) content of impact;4. Soluble semen raphani alkaloids effects on the ApoE-/- mice aorta of nuclear factor-κB (NF-κB) content of aortic endothelial nitric oxide synthase (eNOS) content in thoracic aorta endothelial nitric oxide synthase (eNOS) mRNA expression in impact.The results showed that:1. Soluble semen raphani alkaloids could reduce the ApoE-/- mice with serum triglyceride, total cholesterol, low-density lipoprotein cholesterol, increased high-density lipoprotein cholesterol;2. Soluble semen raphani alkaloids could regulate ApoE-/- mice production and release of vasoactive substances, elevated serum NO content and NOS activity increased serum;3. Soluble semen raphani alkaloids could anti-oxidation, improve the ApoE-/- mice serum SOD activity and lower MDA content;4. Soluble semen raphani alkaloids could inhibit the ApoE-/- mice with aortic protein expression of NF-κB; increase in aortic eNOS protein expression, increasing thoracic aorta eNOSmRNA expression of a protective role in endothelial cell function.Through the research projects, from regulating blood lipids and activation of NO-NOS system on Radish Seed mechanism of action was discussed for the clinical application of Radish Seed treatment of atherosclerosis to provide an objective basis.