The Research On Gene Polymorphisms Related To Power Performance And Predictive Model In Chinese Elite Athletes

Objects: To find the gene markers related to elite power performance,we will explore the association between the single-nucleotide polymorphisms of several candidate gene loci and power performance in Chinese elite athletes.The relationship between genes screened and power performance will be discussed.According to the genes screened,it is explored that polygenetic profiles effect on power performance and the genetic predictive model related to power performance will be established.Methods:(1)Collect the samples of oral mucosal cells in elite athletes,genotype 20 candidate gene loci including ABCC8(rs5210)?ACE(rs4646994)?ACTN3(rs1815739)with SNa Pshot SNP genotyping technology.Association analysis was done between SNPs of candidate genes and power performance in elite athletes.(2)Analyze the relationship between the SNPs data and standing long jump?standing vertical jump?evaluation on power performance by coach in power group athletes.(3)According to the genes and their genotype score,the predictive model based on total genotype score(TGS)will be established.Analyze the effects of polygenetic profile on power performance in Chinese elite athletes.TGS data will be taken back substitution and authenticated in adolescent athletes.Results:(1)Genotype distribution and allele frequency of ACTN3(rs1815739)?ADRB3(rs4994)and CNTFR(rs3808871)were significantly different between power athletes and endurance athletes,controls(P<0.01,P<0.05).Genotype distribution of VDR Apal(rs7975232)was significantly different between power athletes and endurance athletes,controls(P<0.05),yet the allele frequency was not significantly different(P>0.05).(2)ACTN3(rs1815739)polymorphism was correlative in different levels with standing long jump and standing vertical jump(r=0.458-0.749;P<0.01)and moderately correlative with evaluation by coach(r=0.417;P<0.01)in power group.Standing long and vertical jump achievements of ACTN3(rs1815739)RR genotype were significantly higher than that of RX+XX genotype both in male and female power athletes(P<0.01,P<0.05).ACTN3(rs1815739)genotype distribution of excellent grade athletes from coaches' evaluation was significantly different from non-excellent grade athletes(P<0.01).(3)The predictive model based on total genotype score(TGS)was including three gene loci of ACTN3(rs1815739)?ADRB3(rs4994)and CNTFR(rs3808871).The calculation formula was TGS=(100/6)×(GSACTN3+GSADRB3+GSCNTFR).TGS of power group was significantly higher than that of endurance group and control group(P<0.01).TGS was correlative in different levels with standing long jump and standing vertical jump of male and female athletes in power group(r=0.472-0.676;P<0.01).TGS of power group was weakly correlative with their coach evaluation(r=0.300;P<0.05).TGS of adolescent athletes was significantly lower than power athletes(P<0.01)and higher than controls(P<0.01).TGS of adolescent athletes was correlative in different levels with standing long and vertical jump and coach evaluation(r=0.288-0.572;P<0.01,P<0.05).Conclusion: The gene loci of ACTN3(rs1815739)? ADRB3(rs4994)and CNTFR(rs3808871)are associated with power performance in Chinese elite athletes.ACTN3(rs1815739)polymorphism has influence on the variance of phenotype index related to power performance in Chinese elite athletes.The polygenetic profile in our study is associated with power performance in Chinese elite athletes and the predictive model of power performance based on total genotype score has been established.