Studies On Submerged Fermentation Conditions And Extracelluar Polysaccharide' S Physical And Chemical Property Of Pleurotus Ferulae Lanzi

This paper included mainly three aspects: screening of liquid culture medium of Pleurotus ferulae Lanzi, optimization of fermentation conditions, analyses of physical and chemical properties of EPS, the results of which were as follows:1. By monofactorial and orthogonal test, the optimal composition of liquid culture medium for the mycelia's growth and the producing of EPS were respectively determined. The former was composed by 0.002% VB₁, 3% Glucose, 0. 15% Peptone, 0. 05% (NH4)₂SO₄, 0.3% extract of yeast, 0.05% MgSO₄, 0. 1%KH₂PO₄,0.02%ZnSO₄ , 0. 03%NaCl. The later was composed by 0.002% VB₁, 1% Glucose, 0. 15% Peptone, 0.05% (NH4)₂SO₄, 0.3% extract of yeast, 0.05% MgSO₄, 0. 05%KH₂PO₄, 0. 01%ZnSO₄, 0. 03%NaCl.2. The optimal flask (250ml) fermentation conditions were as follows: culture volume was 100mL, inoculum quantity was 10%, initial pH value was 5.0, culture temperature was 25℃, rotation speed was 150rpm, strain age of spawn was 5 days, culture time was 10 days.The optimal jar (50L) fermentation conditions were as follows: culture volume was 30L, strain age of spawn was 5 days, inoculum quantity was 10%, initial pH value was 5.0, culture temperature was 25 ℃, rotation speed was 150rpm, ventilation quantity was 1. 2m³/h , pressure inside the jar was 0. 04Mpa , culture time was 108 hours.3. The result of DEAE-cellulose elution indicated that there was one neutral polysaccharide and one glycoprotein in the water-soluble EPS.4. Analysed with gas chromatography the monosaccharide composition and molecular ratio of water-fast EPS was gained: rha:glu = 4. 08:5. 55, and 4 unknownmonosaccharide.