An Experimental Study Of Therapeutic Effect Of FDP On ARDS Induced By Oleic Acid In Rats

Objective: Acute respiratory distress syndrome (ARDS) is a kind of acute advancing respiratory failure caused by many reason, specialized in respiratory distress and hypoxemia. We made the model of ARDS induced by oleic acid in rats and applied FDP in them to observe the change of lung W/D and the change in morphopathology. We also detected TNF-α &IL-1β in serum, the content of SOD& MDA in lung and the activity of Na~+-K~+-ATPase in lung to explore the therapeutic mechanism of FDP.Method: Divided 30 healthy Wistar rats (weighed 200-250g) into three groups randomly (n=10).1.Control group: Anesthetize rats with 20%urethane (1.0g/kg) through abdomen. Separate one side femoral vein and artery. And then inject normal saline (NS, 2.1 ml/kg).2.Model of ARDS group: After anesthesia and disconnection like above, inject oleic acid (0.1ml/kg) and NS (2.0ml/kg).3.Treatment group in FDP: After anesthesia and disconnection like above, inject oleic acid (O.lml/kg) and FDP (400mg/kg, diluted in 2.0ml/kg of NS).Then three hours later draw blood through abdominal aorta to obtain serum for TNF- α &IL-1β .Take out all lung to observe the change of shape. Then cut right lung for optics microscope and transmission electric microscope, and for detecting SOD, MDA and the activity of Na~+-K~+- ATPase. Weigh, dry and weigh again the left lung.Result:1.The index of biochemistry and the left lung W/D: The model of ARDS group showed the left lung W/D and the content of MDA higher than the control group, meanwhile the content of SOD and the activity of Na~+-K~+-ATPase in lung lower than the control group obviously<0.01.The content of TNF- a &IL-1...