Studies Of KLK 6/KRT 13 Gene In Oral Verrucous Carcinoma

Oral verrucous carcinoma is different from oral squamous cell carcinoma, and it is a kind of disease which is unique in pathological features and biological behaviors. However the pathogenesis of oral verrucous carcinoma is unclear. Based on our previous study about differentially expressed genes between oral verrucous carcinoma and normal mucosa by using DNA methylation chip and genome-wide chip, we selected the target genes for further study. In present study, we investigated the expression of KLK 6 and KRT 13 genes in oral verrucous carcinoma by RT-PCR and immunohistochemistry technique. It will help us in the further study on the molecular mechanism of oral verrucous carcinoma and the target genes for its diagnosis and therapy.ObjectiveTo validate the expression of differentially expressed genes in DNA methylation chip and genome-wide chip and study the expression of KLK 6 and KRT 13 genes in oral verrucous carcinoma.MethodsWe collected the tissue of oral verrucous carcinoma and normal mucosa from 4 patients to study the expression of KLK 6 and KRT 13 genes at mRNA level by using RT-PCR. With immunohistochemical technique, we uncovered the differential expression of KRT 13 protein in 19 patients of oral verrucous carcinoma,26 patients of squamous carcinoma and 14 of normal mucosa tissue. ResultsThere was not statistically significant (P<0.05) in the expression of KLK 6 in the tissues of oral verrucous carcinoma, para-carcinoma and the normal. There was statistically significant (P<0.05) in the expression of KRT 13 between the tissues of oral verrucous carcinoma and the normal. There was not statistically significant (P<0.05) in the expression of KRT 13 between the tissues of oral verrucous carcinoma and para-cacinoma. There was statistically significant (P<0.05) in the expression of KRT 13 between the tissues of para-cacinoma and the normal. There was statistically significant (P<0.05) in the expression of KRT 13 protein in the the tissues of oral verrucous carcinoma, squamous carcinoma and the normal. There was statistically significant (P<0.05) in the expression of KRT 13 protein in the tissues of exophytic type of oral verrucous carcinoma, cyst type of oral verrucous carcinoma, infiltration type of oral verrucous carcinoma and undifferentiated squamous carcinoma. There was statistically significant (P<0.05) in the expression of KRT 13 protein in the the tissues of well differentiated and moderate-low differentiated squamous carcinoma. There was not statistically significant (P<0.05) in the expression of KRT 13 protein in the the tissues of well-moderate differentiated and the exophytic type of oral verrucous carcinoma. There was not statistically significant (P<0.05) in the expression of KRT 13 protein in the tissues of moderate-low differentiated squamous carcinoma, the cyst type of oral verrucous carcinoma and infiltration type of oral verrucous carcinoma.Conclusion1. The expression of KRT 13 mRNA was at low level in oral verrucous carcinoma. The result was as same as the results of previous gene chips. The expression of KRT 13 protein in oral verrucous carcinoma was lower than in the normal mucosa tissues. KRT 13 might be a functional factor in the initiation and development of oral verrucous carcinoma. There was not statistically significant in the expression of KLK 6 mRNA in the tissues of oral verrucous carcinoma and the normal. Further study on the function of KLK 6 mRNA in oral verrucous carcinoma was required.2. The expression of KRT 13 protein in oral verrucous carcinoma was related to the clinical classification.3. KRT 13 protein can be used as the prognosis factors of oral verrucous carcinoma, and offer the theory support for gene therapy of oral verrucous carcinoma.