| Non-alcoholic fatty liver disease is a type of liver tissue pathology clinical syndrome. And alcoholic liver disease similar to, but no history of excessive drinking. Because of their prevalence is high, the occult, hepatic fibrosis and cirrhosis and other developmental characteristics. and on the prevention and treatment of NAFLD have been affected by the widespread interest both at home and abroad. About the pathogenesis of fatty liver, numerous researchers unanimously and adiponectin (Adi) and leptin (Lep), leptin receptors have a certain contact. .Lep is produced by fat cells and a protein. In a normal physiological situations, Lep quantities, can be fed back to the central system, regulating hypothalamus parasympathetic nervous system, to accommodate the hunger or feeding of reaction[1] adiponectin is a unique source of adipose tissue to polypeptides, its body obesity Downregulation of research tips, adiponectin and leptin in the development of NAFLD played an important role [2] [3].The body of the hypothalamic area is the body of the endocrine and metabolic activity of important structural areas. In particular, the hypothalamus-neuropeptides pathway in the feeding of the feedback loop into play. Leptin and leptin receptors is the Messenger of the incoming signal to the full in the hypothalamus melanin-4 receptors and their ligands melanin-melanocyte-stimulating hormone leptin, and play a key role in the courier and rely on the hypothalamus regulation effect. A large number of laboratory confirmed, electro-acupuncture can be adjusted with the endocrine system, this is the mechanism of the effect of acupuncture. Electroacupuncture NAFLDA has been a large number of experiments confirmed, about its mechanism of action through hepatic cytochrome expression and antioxidant effects for oxidation treatment of [4].Acupuncture as a natural therapy, has been incorporated into the world of medicine. At the same time, the mechanism of acupuncture is also widely appreciated. Therefore this study by detecting electroacupuncture of rats detection leptin, adiponectin, and other related indicators, on treatment of electroacupuncture NAFLD.Objective:Inspection body adiponectin levels and hypothalamus leptin (Lepton, Lep), its receptor gene expression (mRNA), on the treatment of non-alcoholic fatty liver disease (nonalcoholic fatty liver disease, NAFLD) of the mechanism of action.MethodThe distant Sprague Dawley rats (Group, SD) 32 random to model group 22 only and empty group 10 only. The normal feed blank group, high fat diet fed model preparation SD rat model group. 8 week will randomly divided into rat model group: a control group (11 only), acupuncture Group (11 only). Treatment of acupuncture group, the "comments", "in", "vaginal mausoleum Springs", "Taichong", "over", and other points to acupuncture and frequency 10 Hz, strength of 1. 5 v CW; treatment of 4 weeks, each group of serum triglyceride (TG), Trglyceride, serum cholesterol (serum cholesterol, TC), serum Lep, adiponectin, observation histopathological changes in the liver, and RT-transfer amine by chain polymerization (Reverse Transcription-Polymerase Chain Reaction, RT-PCR) method for detecting hypothalamus Lep and its receptor mRNA expression.Results1 outward appearance:Each group of rats in the experiment does drinking water during feeding, drinking water, food consumption without obvious exception. The control group of rats fur glossy, posture and lively; model group rats obviously increase, body fat, skin discoloration, apparently not. An empty set of rat liver Visual inspection is bright red, edge sharpness, smooth surface. Model group see rat liver enlargement with the naked eye, edge blunt, washed Khaki, slice is greasy. Join the mouse after making photos2 HE dyeing show: Model group rats: hepatocytes cytoplasmic high levels of fat vacuoles are clear, the nucleus is fat vacuoles crush into liver cell margin, pathology confirmed fatty liver has formed. Electroacupuncture group treatment, liver fatty tissue denaturation far, liver cell structure also tend to be normal.3 Leptin,adiponectin indexThe treatment group and model group compared liver tissue homogenate leptin levels markedly. Liver tissue homogenate adiponectin levels have significantly lower serum TG TC, a marked decline.4 RT-PCRRT-PCR Detection shows: model group rats Lep and its receptor mRNA are noticeably, electro-acupuncture group hypothalamic Lep and leptin receptors increases. Lep optical density Analyzer on NAFLD hypothalamic, its receptor mRNA expression comparison table 2).ConclusionAfter eight weeks of fat rat feeding NAFLD have been making success. Experimental electro-acupuncture can influence hypothalamic Lep, its receptor mRNA expression, mitigation fat metabolism disorders, reached therapeutic effect on NAFLD, speculated that the treatment of non-alcoholic fatty liver disease mechanisms through "effect" effect to neuro-endocrine regulation system, adjustment of the Lep, its receptor mRNA expression and adiponectin levels.
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