| Our work focused on the correlation between the recruitment of fructose-1,6 -diphosphate(FDP) and the activity of enzymes and metabolism of free radicals in myocardia and serum of rats that were exhausted by increasing the load of exercise to cause exercise-induced fatigue. The experimental results supported that FDP could delay the exercise-induced fatigue and facilitated the removal of fatigue. Also, we believed that the myocardia of rats functioned as protectors.90 male rats were randomly divided into 9 groups, they are untrained group (UT) with 10 rats, trained control group (TC) with 40 rats randomly divided to 4 subgroups (TC1, TC2, TC3 and TC4), and medicine-treated trained group (TT) with 40 rats randomly divided to 4 subgroups (TT1, TT2, TT3 and TT4). TC and TT groups were trained with increasing load of treadmill running for 7 weeks till exhausted in the last training. TT groups received intrapearitioneal(i. p.) injection with 1g/kg of body mass of FDP immediately after training each time. FDP was injected 50 minutes before the last training. TC groups were i.p. injected with saline of the same volume accordingly. Myocardia and serum of 5-weeks later, immediate after exhaustion, 6 hours and 24 hours after exhaustion were collected and superoxide dismutase(SOD), lactate dehydrogenase(LDH), creatine kinase(CK) and malondialdehyde(MDA) were measured. Same parameters were also measured in the UT group. The results turned out to be as follows:(1) After 5-weeks' increment of exercise load from low to medium intensity, activity of myocardial CK and LDH increased while serous CK and LDH decreased in both TT and TC groups, compared with UT group. But there is no significant difference between TT and TC. Right after exhaustion, activity ofmyocardial CK and LDH achieved maximal value in TT, which was significantly higher than that in TC. While activity of serous LDH and CK increased remarkably in TT, but it is much lower than in TC. It suggested that aerobic exercises at the low or medium intensity could help to improve the capability of energy supply in myocardial tissue. FDP showed some protecting function to myocardia in rats with exercise-induced fatigue.(2) After 5-weeks' increment of exercise load from low to medium intensity, expression level of MDA in both myocardia and serum showed a trend to decrease, while activity of SOD and the ratio SOD/MDA appeared to be increasing, with no significant difference between TT and TC. Right after exhaustion, level of MDA and activity of SOD both increased in myocardia and serum, while SOD/MDA decreased drastically. TT has a higher MDA level and lower SOD activity and SOD/MDA ratio than TC, indicating that FDP was well resistant to lipid peroxidation.(3) This study emphasized on the regularities of myocardial and serous CK, LDH, SOD and MDA in different recovery phases of incremental exercise-induced rats when recruited FDP. Compared with TC, activities of CK, LFH and SOD in myocardia were enhanced while MDA was reduced in the exhaustion and the following recovery phases in TT. In serum, activity of CK and LDH were reduced, while SOD activity and SOD/MDA increased. All the facts indirectly proved that FDP could delay the exercise-induced fatigue and facilitate the removal of fatigue. |
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