| Objective:This research tested exercise ability,free radical,antioxidant and antioxidant enzyme in the brain of big rats which drunk the traditional Chinese medicine for benefiting kidney and profiting spleen and trained, through analyzing the change of above indexes to explore the relation between free radical and exercise-induced central fatigue and the mechanism of traditional Chinese medicine preparation resist exercise-induced central fatigue. In order to provide the scientific theory basis to guide sports training and exercises to keep in good health, to eliminate exercise-induced fatigue and enhance exercise ability, to make anti-fatigue traditional Chinese medicine preparation.Method:Selected 120 healthy male Sprague-Dawley big rats which were 8-week-old, the big rats were randomly divided into contrast group (group C), drinking medicine group (group M), training group (group T), training and drinking medicine group (group TM). The big rats drunk traditional Chinese medicine in group M and group TM and drunk pure water in group C and group T, about 1ml/100g body weight, once/one day; The big rats carried out progressive resistance swimming training five weeks in group T and group TM, the big rats trained six days and rested one day every week. The kidneys were taken from the body of big rats after five weeks. Each group rats were divided into three inferior groups again according to the state (quite, exhaustion immediately, recover 12 hours after exhaustion) which were executed, tested the content of NO,MDA,GSH and the activity of SOD in the brain of rats respectively. All dates were analyzed by SPSS13.0.Results:1 In the whole course of experience, four groups big rats are invigorating and lively, their fur is bright and tidy, their eyes are shining; no below the symptom appears, such as tired and sleepy, eyes faint, narrow eyes and bow back, body thin and weak, fur disorderly and no light, even fur drop. Each group big rats' weight is on the trend of steady groeth.2 Compare with group C, the time of other three group big rats swim to exhaustion is obvious prolong, and this phenomenon is most obvious (P<0.01) in group TM.3 Under the state of quiet, the change of NO and MDA content is consistent, compare with group C, the content all reduce in other three groups, the range of decrease is biggest in group TM; compare with group C, the activity of SOD both reduce in group M and group TM, the activity of SOD rise in group T; compare with group C, the content of GSH all reduce in other three groups.4 Under the state of exhaustion immediately, compare with group C, the content of NO and MDA all reduce in group M,group T,group TM; compare with group C, the activity of SOD reduce and the content of GSH reduce in group M and group TM, the activity of SOD rise and the content of GSH increase in group T.5 Under the state of recovering 12 hours after exhaustion, the change of NO and MDA content is consistent, compare with group C, the content all reduce in other three groups, the range of decrease is biggest in group TM; compare with group C, the activity of SOD rise a bit in group M and reduce a bit in group T, but those change hasn't significance in statistics, the activity of SOD obviously reduce in group TM; compare with group C, the content of GSH rise in group M and reduce in group T and group TM.Conclusion:1 The traditional Chinese medicine for benefiting kidney and profiting spleen and training can enhance the exercise ability of big rats effectively, prolong the time of big rats swim to exhaustion obviously, and the result of drinking medicine and combine training is better.2 The traditional Chinese medicine for benefiting kidney and profiting spleen and training make the free radical NO and the outcome of lipid peroxidation MDA reduce obviously, and the range of decrease is biggest in drinking medicine and combine training group, those indicate that the traditional Chinese medicine preparation and training can inhibit the reaction of lipid peroxidation, reduce the generation of free radical NO in the brain.3 The change of SOD activity and GSH content which are influenced by the traditional Chinese medicine for benefiting kidney and profiting spleen and training didn't show specified regulation in the brain of big rats in this experience, the mechanism that the traditional Chinese medicine for benefiting kidney and profiting spleen and long-period moderate intensity progressive resistance training influence the antioxidant and antioxidant enzyme remain need discussed further. |
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