Effect Of Aqueous Extract Of Rhizoma Polygonati On The Metabolism Of Free Radicals In Brain Tissue Of Mice Under Different Conditions

1Objective:By observing the influence from aqueous extract of polygonatum on exercise training miceof different state under the function of brain tissue T-SOD?total-superoxide dismutase??T-AOC(total antioxidant capacity)?GSH (glutathione) and GSH-PX?glutathione peroxidase?and MDA?malondialdehyde?content affects, this paper is to explore the impact of aqueous extract ofpolygonatum which could improve the antioxidant mechanism of action and resistance to fatigue,and better service for sports practice.2Method:Kunming mice, male, are offered by Shandong Lukang Pharmaceutical AnimalExperiment Center160, each weighing about25g±2g. The animal room temperature was23-28?, humidity was40%-65%, and ventilation was good. Fed by mixed forage and cold waterrandomly and controlled strictly, the mice were divided into cages and10in each cage. Afterone-week adaptation feeding in the animal room, the mice were randomly divided into4majorgroups, including control group, medication group, training group, training+medication group,each group was30, the total was120. Based on the death state, each group was further dividedinto four subgroups: the quiet, quantitative movement1h, the exhaustive status and recoveryafter exhaustive exercise12h, decapitated and the brain was determination of the relevant,indicator at the appropriate moment. All data are used SPSS17.0statistical software to analyze.3Result:3.1Exhaustive time, the exhaustive time of the medication group, the training group and thetraining+medication group were longer than the control group (P<0.05); the training+medication group was significantly longer than the training group and medication group(P<0.05).3.2In rest state?T-SOD activity, medication group, exercise group and training+medicationgroup were significantly higher than that in the control group (P<0.05), and the training+medication group was significantly higher than the training group and medication group(P<0.05); T-AOC activity, medication group, exercise group and training+medication groupwere significantly higher than that in the control group (P<0.05), the exercise group wassignificantly higher than medication group?P<0.05?; and the training+medication group wassignificantly higher than the training group and medication group (P<0.05); GSH content andGSH-PX activity, medication group, exercise group and training+medication group weresignificantly higher than that in the control group (P<0.05), and the training+medication groupwas significantly higher than the training group and medication group (P<0.05); the content ofMDA, medication group, exercise group and training+medication group were significantly lower than that in the control group (P<0.05), the training+medication group was significantlylower than the training group and medication group (P<0.05).3.3Movement1h, T-SOD activity and T-AOC activity, medication group, exercise group andtraining+medication group was significantly higher than that in the control group (P<0.05), andthe training+medication group was significantly higher than the training group and medicationgroup (P<0.05); GSH content and GSH-PX activity, medication group, exercise group andtraining+medication group was significantly higher than that in the control group (P<0.05), thetraining+medication group was significantly higher than the training group and medicationgroup (P<0.05); the content of MDA, medication group, exercise group and training+medication group was significantly lower than that in the control group (P<0.05), the training+medication group was significantly lower than the training group and medication group(P<0.05).3.4Exhaustive exercise immediately, the activity of T-SOD, medication group, exercise groupand training+medication group was significantly higher than that in the control group (P<0.05),the training+medication group was significantly higher than the training group and medicationgroup (P<0.05); T-AOC activity, medication group, exercise group and training+medicationgroup was significantly higher than that in the control group (P<0.05); GSH content, medicationgroup, exercise group and training+medication group was significantly higher than that in thecontrol group (P<0.05), the exercise group was significantly higher than medication group?P<0.05?, the training+medication group was significantly higher than the training group andmedication group (P<0.05); GSH-PX activity, medication group, exercise group and training+medication group was significantly higher than that in the control group (P<0.05), the training+medication group was significantly higher than the training group and medication group(P<0.05); the content of MDA, medication group, exercise group and training+medicationgroup was significantly lower than that in the control group (P<0.05), the training+medicationgroup was significantly lower than the training group and medication group (P<0.05).3.5At the12hours ends of exhaustive exercise, the activity of T-SOD, medication group,exercise group and training+medication group was significantly higher than that in the controlgroup (P<0.05), and the training+medication group was significantly higher than the traininggroup and medication group (P<0.05); T-AOC activity, medication group, exercise group andtraining+medication group was significantly higher than that in the control group (P<0.05); thetraining+medication group was significantly higher than the training group and medicationgroup (P<0.05); and other group was no significant difference?P<0.05?; GSH content andGSH-PX activity, medication group, exercise group and training+medication group wassignificantly higher than that in the control group (P<0.05), the training+medication group was significantly higher than the training group and medication group (P<0.05); the content of MDA,medication group, exercise group and training+medication group was significantly lower thanthat in the control group (P<0.05), the training+medication group was significantly lower thanthe training group and medication group (P<0.05).4Conclusions:4.1Taking the aqueous extract of polygonatum or swimming can improve brain tissue in mice ofdifferent condition, T-SOD activity, T-AOC level, GSH content and GSH-PX activity anddecreased MDA content, and enhance brain tissue free radical scavenging ability,4.2Taking the aqueous extract of polygonatum and swimming a combination can significantlyimprove the exercise capacity of mice, and enhance the different functional status in mice's braintissue T-SOD activity, T-AOC level, GSH content and GSH-PX activity and reduced MDAcontent than simple taking the aqueous extract of polygonatum or simple swimming better.