| OBJECTIVE:Hypertension is a common chronic disease.The traditional Chinese medicine has obvious advantages in improving the symptoms of hypertension and the quality of life.Prefessor Qian Linchao,after many years of clinical experience,has sunned up that one of the major pathogenesis of hypertension is stagnation of liver qi and excessive fluid.Therefore,we use Chailing Jiangya Decoction to treat such patients with hypertension in clinical.This decoction plays an important role in controlling blood pressure and improving symptoms by Liver-soothing and Urination-promoting.The paper aims to clear the mechanisms of Chailing Jiangya Decoction regulating hypertension and left ventricular hypertrophy by observing the effect of the decoction on blood pressure,left ventricle and oxidative stress in SHR,and provide a scientific evidence for the clinical application of it.METHODS:40 SHR were randomly divided into 4 groups:model group,telmisartan group(7 mg/kg),high-dose(57.6g/kg),and low-dose Chailing Jiangya Decoction group(14.4 g/kg);10 WKY rats were taken into normal control group.The model group and WKY group were given the same amount of distilled and the other groups were treated with different drugs for 6 weeks.(1)We measured the blood pressure respectively before treatment and 2weeks,4weeks and 6 weeks after treatment.(2)After 6 weeks,by Enzyme-linked immunosorbent assay(ELISA),we investigated the detections of eNOS,PGI2,EDHF,ET-1,ROS and Ang? in serum of rats.(3)After 6 weeks,we weighed the qualities of body mass and left ventricle mass of rats and counted the LVMl.By ELISA,we measured the detections of Sirt3,pAMPK and pACC in the left ventricle.By real-time PCR,we detected the relative quantifications of Sirt3 mRNA,AMPK mRNA and ACC mRNA in the left ventricle.(4)By Hematoxylin-eosin(HE)staining,we made pathological sections to observe the left ventricle of rats.RESULTS:(1)The blood pressure of model group increased with age.During the treatment,the blood pressure of high-dose Chailing Jiangya Decoction group showed no obvious fluctuation and then decreased obviously,while there was no significant fluctuation in low-dose one.(2)After 6 weeks,in the endothelial derived relaxing factors,compared with the model group,the detections of eNOS,PGI2 and EDHF in the High-dose Chailing Jiangya Decoction group and EDHF in the Low-dose one was increased,while eNOS and PGI2 in the Low-dose one showed no obvious change.In the endothelial derived contracting factors,the detections of ET-1,ROS and Ang ? in the High-dose group and ET-1,Ang ? in the Low-dose one was reduced,while ROS in the Low-dose one had no obvious change.(3)After 6 weeks,in the high-dose group,the LVMI decreased,the detections of Sirt3 and pAMPK increased,and pACC,Sirt3 mRNA,AMPK mRNA and ACC mRNA showed no obvious changes.While there were no significant effects in the low-dose one.(4)In the high-dose group,the detection of ROS reduced,while eNOS,PGI2,EDHF,Sirt3 and pAMPK which can inhibit oxidative stress in SHR increased.(5)HE staining results:compared with the model group,in the high-dose group,the arrangement of myocardial cells were more regular,and the myocardial cells of Low-dose one were not significantly improved.CONCLUSIONS:(1)The Chailing Jiangya Decoction can prevent the rise of the blood pressure,and even lower the blood pressure in SHR.(2)The Chailing Jiangya Decoction can improve endothelial function in SHR,and the mechanism may be related to the increase of endothelium-derived relaxing factors and the decrease of endothelium-derived contracting factors.(3)The Chailing Jiangya Decoction can improve left ventricular hypertrophy in SHR,and this may be related to the activation of Sirt3-AMPK pathway.(4)The Chailing Jiangya Decoction attenuate oxidative stress in SHR,and this may be one of the reasons of improving of blood pressure and left ventricular hypertrophy. |
PDF Full Text Request