Studies On Chemical Constituents Of Psoralea Corylifolia L., Aristolochia Contorta And Valeriana Jatamaisi Jones

The thesis mainly reported chemical and DNA cleavage constituents from three traditional herbs Psoralea corylifolia L, Aristolochia contorta and Valeriana jatamansi Jones.Psoralea corylifolia L. belongs to Leguminosae and possesses efficiency of tonifying kidney, strengthening yang, warming spleen and stopping diarrha. Up to now, chemical constituents of the plant were focused on coumarins and flavours. Psoralen, the main constituent could inhibit growth of mice sarcoma and liver cancer. Other compounds also showed pharmacological effects.In our search of plant derivated DNA cleavage agents, ethanol extract of Psoralea corylifolia was found to show strong DNA cleaving activity. By the combination of various separation technique, including silica gel chromatography, reversed phase silica gel chromatography, Sephadex LH-20 chromatography and polyamide chromato- graphy, we isolated 15 compounds from this herb. 14 compounds were identified by spectroscopic methods to be as psorachalcones A(1), B (2), isobavachalcone (3), bavachalcone (4), bavachromene (5), bavachinin (6), bavachin (7), neobavaisoflavone (8), corylin (9), psoralen (10), isopsoralen (11), psorilidin (12) , pinitol (13) and p- hydroxy-benzaldehyde (14). Psorachalcones A(l) and B (2) were two new compounds and pinitol (13) was isolated from the plant for the first time. Psorachalcones A (1) and B (2) displayed strong DNA strand scission activity.Aristolochia contorta belongs to the family Aristolochiaceae and distributes over tropic and subtropic areas. 200 species of Aristolochia were distributed in the world and more than 40 species in China, which could be used to relieve pain and cough, prevent asthma and diminish inflammation. In our search of plant derivated DNA cleavage agents, ethanol extract of Aristolochia contorta was found to show strong DNA cleaving activity. By the combination of various separation technique, weisolated 12 compoumds from this plant. 10 of them were identified by spectroscopic methods to be as 3"-hydroxy-amemtoflavone (a-1), 3"-hydroxy- amemtoflavone-7-O-mether ether (a-2), 2", 3"-dihydro-3"-hydroxy-amemtoflavone (a-3), aristolo-chic acid Iva (a-4), 7-methoxyaristolochic acid (a-5), aristolactam-N-B-D- glucopy-anoside (a-6), aristoloctam la N-B-D-glucopyanoside (a-7), 6,7,11,16, 17-pentahydroxy-kaurane-19-oic acid (a-8), daucosterol (a-9) and pinitol (a-10). Compounds a-l~ a-3 were new compounds and showed significant DNA cleavage activities.The genus Valeriana (Valerianaceae) comprises about 200 herbs and shrubs throughout world. Valeriana jatamansi Jones grows in dump organic soil over the whole Yunnan province, Sichuan, Guizhou, Hubei, Henan and Shaanxi, which was used to help digestion, regulate the flow of vital energy and remove obstraction toit, treat diarrhoea and relieve rheumatic pains. In our search of plant derivated DNA cleavage agents, ethanol extract of Valeriana jatamansi Jones was found to show strong DNA cleaving activity. In order to find new active compounds, we study chemical constinents of Valeriana jatamansi. Twenty compounds were isolated. Among them, twelve were identified by spectroscopic methods to be as 11-methoxy viburtinal (v-1), baldrinal (v-2), valerioates A (v-3), B (v-4), C (v-5), IVHD-valtrate (v-6), valerosidate (v-7), 8, 8'-dihydroxypinoresinol-4'-O-B-D- glucoside (v-8), coniferin (v-9), hexacosanic acid (v-10), daucosterol (v-11) and -sitosterol (v-12). 11- methoxy viburtinal (v-1), valerioates A (v-3), B (v-4), C (v-5) and 8, 8'-dihydroxy-pinoresinol-4'-O-B-D- glucoside (v-8) were new compounds. Coniferin (v-9) was isolated from this plant for the first time. All compounds showed poor DNA splitting action.At last, advance on chemical constituents of three plants were reviewed including compounds, structures, source plants and references.