Effect Of Yishen Capsule On SFRP-1 In Renal Tubule-interstitium Of Rats With Diabetic Nephropathy

Objective: To investigate the effect of Yishen Capsule on sFRP-1 in renal tubule-interstitium of rats with diabetic nephropathy.Methods: The rats models of diabetic nephropathy were established by injecting withstreptozotocin intraperitoneally. Wistar male rats were randomly divided into 4 groups: thecontrol group, the diabetic nephropathy model group, the Yishen Capsule treated group andlosartan treated group, each group have 8 rats. The rats in Yishen Capsule treated group weregiven Yishen Capsule 625mg/kg through intragastric administration once daily for 12 weeks. Therats in each group were scarificed at the end of 12th week after model established. Blood glucose,blood serum creatinine, blood ureanitrogen, 24-h urinary protein were measured, kidneys wereprocessed for HE-stain, PAS-stain as well as immunohistochemistry to observe morphologicalchanges and the expression of sFRP-1,β-catenin; real-time fluorescent quantitative PCRanalyses the expressions of sFRP-1 mRNA,β-catenin mRNA.Results: 1. At the end of 12th week, the expressions of sFRP-1,β-catenin in cytoplasm ornucleus, sFRP-1 mRNA,β-catenin mRNA were significantly increased in the diabeticnephropathy model groups, which was accompanied by higher levels of blood glucose, 24-hurinary protein, Scr, BUN and pathology changes compared with the control group(P<0.05). 2.After treatment of Yishen capsule, the expressions of sFRP-1, sFRP-1 mRNA up-regulatedfurther, the expressions ofβ-catenin in cytoplasm or nucleus,β-catenin mRNA and the 24-hurinary protein, Scr, BUN, pathology changes significantly attenuated compared with thediabetic nephropathy model groups.Conclusion: The Yishen capsule could up-regulated the expression of sFRP-1 in renaltubule-interstitium andβ-catenin was inhibited, prevented fibrosis of renal tubular epithelial ofrats with diabetic nephropathy.